Chimeric antibodies are usually too immunogenic to be used as potential therapies in humans. Humanization is the process to reduce immunogenicity and improve human immune activation against the antibody in a necessary preclinical step. Using comprehensive design software and advanced technologies including canonical scaffold simulation and 3D modeling, ABclonal assures the humanized antibody retains functional activity comparable to the parental antibody.

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Antibody Humanization Service Process

Phase I

  • Sequence analysis, chimeric antibody construction and humanized antibody design

Phase II

  • Gene synthesis, molecular cloning, and evaluation of humanized variants

Phase III

  • Design of back mutations

Phase IV

  • Evaluation of introduced back mutations

Phase V

  • Final humanized antibody expression, purification, and characterization
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Complete Package Details

Standard package includes:

  • Antibody sequencing, chimeric antibody generation and characterization
  • Stability assessment of chimeric antibody
  • Humanization design and sequence analysis
  • Lead human antibody optimization
  • Final KD validation using BiacoreTM

Additional service:

  • SDR (specificity determining residue) for super-humanized antibody
  • Cysteine engineering

Deliverables:

  • Written report at every stage
  • At least one humanized antibody with less than 3-fold loss of antigen binding affinity
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