You may have an existing hybridoma that produces your valuable monoclonal antibody reagent or wish to ensure continued production of the customized antibody you generated with ABclonal. To help you maintain your critical research antibody, we offer antibody expression and sequencing services, either in coordination with our standard service or using your provided hybridoma, so you can have a steady supply of your reagent for future projects.
Antibody Expression Package
Light and heavy chain gene synthesis
ELISA validation of monoclonal antibody
Expansion and purification of antibody
- Gene synthesis – light chain and heavy chain provided by the customer
- Subclone into ABclonal proprietary expression vector
2-3 WEEKS
Express & validate
- Small-scale express antibody in CHO
- Validate recombinant antibody using ELISA
- Send sample to the customer to validate
2-3 WEEKS
Expand and Purify
- Expand and purify antibodies in 1 L of culture fluid
- Protein A/G or affinity purification (the yield is dependent on the nature of the antibody)
2 WEEKS
Select High-expression
- Select high-expression CHO cell line (optional) CHO cells
- >100 mg/L
Antibody Sequencing Service
Knowing the antibody sequence is essential for monoclonal antibody engineering, antibody humanization, functional optimization, and more. ABclonal provides customers with high quality hybridoma sequencing services in addition to other deep sequencing services to yield the exact variable region sequences for downstream applications for your antibody.
Highlights
Sequence accuracy
Fast and economical service
Broad experience with multiple species, including human, mouse, and rat
Starting from a hybridoma cell line, we offer the following services
Complete Package Details
Samples
- Customer should provide at least two (2) vials of 1 x 106 (or more) hybridoma cells
Total RNA
- RNA concentration no less than 100 ng/μl
- RNA volume no less than 20 μl
- RNA bands will be clear, with no significant degradation
Service Timeline
- Antibody isotyping identification
- 3-5 business days (alternatively, provided by customer)
Variable domain sequencing
Full-length antibody sequencing
Deliverables
- Detailed performance report
- Sequence data
- Alignments of heavy and light chains
- Plasmids containing VH/VL sequences
Note:
Please send us the samples as soon as possible using dry ice or liquid nitrogen. Delivery time will speed up significantly if customers provide the correct isotype and species information.
