Yeast Expression System

The yeast expression system is the most economical eukaryotic expression system for both secreted and intracellular proteins. It is ideally suited for large-scale production of recombinant eukaryotic proteins. Our yeast expression system provides the advantages of both prokaryotic and eukaryotic expression systems: high expression, low cost, ease of scale-up, and proper protein folding. We have multiple strains and expression vectors to help identify the highest yielding conditions. We also utilize robust secretion signal sequences to ensure the highest secretion of recombinant protein. Our customers can also choose from constitutive or inducible expression systems.

Yeast Expression Flowchart

Highlights


Optional hosts
Saccharomyces cerevisiae and Pichia pastoris.
High success rate
The success rate is up to 90%.
Optional vectors
Auxotrophic expression vector for the secretory and intracellular protein provided by ABclonal, and the bacteria strain vector for high efficient screening expression can be transformed to wild type pichia pastoris.
Rich expression experience
Select the best possible expression vector, explore and optimize the conditions based on customs' requirement.
Large-scale production
1-4000L per batch.
One-stop service
From gene to protein.Design and optimize the gene, virus production, express and optimize the protein, process the protein post-purification, deal with the data and write the experimental report for the customer.

Package Details


Service Name Service Content Timeline
Plasmid Construction
  • Gene synthesis with codon optimization& subclone
  • Construct linearization

 

3-5 Weeks
Protein Expression Test
  • Transformation of linear plasmid into suitable host
  • Antibiotic screening, select 5-10 positive clones
  • Expression analysis and identification
  • 100ml amplification and purification test
  • Tag-free test(if required)

 

4-7 Weeks
Large-scale fermentation & purification
  • Protein affinity purification(or other purification method)
  • QC the purified protein
1-10L 3-4 Weeks
10-20L 4-5 Weeks
20-50L 5-6 Weeks
Above 50L 7-14 Weeks

Note:

QC: SDS-PAGE/WB identification of the purified protein,MS identification(if required).

All service timelines are approximate. In most cases, we finish the custom project within the appointed time. However, optional steps might prolong the deliver timeline.

Custom protein service is charged step by step and save your valuable research costs.

Protein Service Quotation Request Form
  • For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  • Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  • Please send your samples to NeoScientific, 86 Cummings Park, Woburn, Massachusetts.
  • Our customer service is available 24hours per day/7 days a week/365 days a year.

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