Product Type > Antibodies > Tag and Loading Control Antibodies

β-Actin Mouse mAb (AC004)

Reviews (2) Publications (338) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - β-Actin Mouse mAb (AC004)

Western blot analysis of lysates from HeLa cells, using β-Actin Mouse mAb (AC004) at 1:2000- 1:640000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - β-Actin Mouse mAb (AC004)

Western blot analysis of various lysates, using β-Actin Mouse mAb (AC004) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - β-Actin Mouse mAb (AC004)

Immunohistochemistry analysis of β-Actin in paraffin-embedded human colon tissue using β-Actin Mouse mAb (AC004) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - β-Actin Mouse mAb (AC004)

Immunohistochemistry analysis of β-Actin in paraffin-embedded mouse testis tissue using β-Actin Mouse mAb (AC004) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - β-Actin Mouse mAb (AC004)

Immunohistochemistry analysis of β-Actin in paraffin-embedded rat spleen tissue using β-Actin Mouse mAb (AC004) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - β-Actin Mouse mAb (AC004)

Immunofluorescence analysis of U2OS cells using β-Actin Mouse mAb (AC004) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for β-Actin Mouse mAb(AC004)
Review
ABclonal: review for β-Actin Mouse mAb(AC004)

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Overview

Product name β-Actin Mouse mAb
Catalog No. AC004
Host species Mouse
Purification method Affinity purification
Isotype IgG1, Kappa
CloneNo. AMC0001

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.

Immunogen information

Immunogen Fusion protein of human β-Actin
Sequence Email for sequence
Gene ID 60
Swiss prot P60709
Synonyms BRWS1; PS1TP5BP1; β-Actin
Calculated MW 42kDa
Observed MW 42kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:2000 - 1:20000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Antibody Dilute Procedure Put 5ul AC004 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples 293T, HeLa, A-549, NIH3T3, Mouse brain, Rat testis, C6
Cellular location Cytoplasm, cytoskeleton
Customer validation

IHC (Mouse kidney, Rat kidneyHomo sapiens, Mus musculus)

WB (Mus musculus, Homo sapiens, Crassostrea gigas, Rattus norvegicus, Citrus sinensis, Sus scrofa, Ctenopharyngodon idellus, Cynoglossus semilaevis, Spodoptera frugiperda, Pinellia ternata, Arabidopsis thaliana, Actinopterygii, insect, Plutella xylostella, Bombyx mori Linnaeus, Lineage, Canis lupus familiaris, Lampetra japonica, Staphylococcus aureus, Danio rerio, Rattusnorvegicus, Gallus gallus, mus musculus, Homo sapiensMus musculus, Ictalurus punctatus, Larimichthys crocea)

IF (Homo sapiens, Mus musculus)

Cellular Uptake and In Vitro Fibrotic Marker Visual-ization (Mus musculus)

co-IP (Homo sapiens)

qPCR (Mus musculus)

IP (Homo sapiens, mus musculus)

Co-IP (Homo sapiens)

CHIP (mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

β-Actin Mouse mAb images

ABclonal:Western blot - β-Actin Mouse mAb (AC004)}

Western blot - β-Actin Mouse mAb (AC004)

Western blot analysis of lysates from HeLa cells, using β-Actin Mouse mAb (AC004) at 1:2000- 1:640000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - β-Actin Mouse mAb (AC004)}

Western blot - β-Actin Mouse mAb (AC004)

Western blot analysis of various lysates, using β-Actin Mouse mAb (AC004) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - β-Actin Mouse mAb (AC004)}

Immunohistochemistry - β-Actin Mouse mAb (AC004)

Immunohistochemistry analysis of β-Actin in paraffin-embedded human colon tissue using β-Actin Mouse mAb (AC004) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - β-Actin Mouse mAb (AC004)}

Immunohistochemistry - β-Actin Mouse mAb (AC004)

Immunohistochemistry analysis of β-Actin in paraffin-embedded mouse testis tissue using β-Actin Mouse mAb (AC004) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - β-Actin Mouse mAb (AC004)}

Immunohistochemistry - β-Actin Mouse mAb (AC004)

Immunohistochemistry analysis of β-Actin in paraffin-embedded rat spleen tissue using β-Actin Mouse mAb (AC004) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - β-Actin Mouse mAb (AC004)}

Immunofluorescence - β-Actin Mouse mAb (AC004)

Immunofluorescence analysis of U2OS cells using β-Actin Mouse mAb (AC004) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on ACTB. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ACTB. (Distance between topics and target gene indicate popularity.) ACTB

* Data provided by citexs.com, for reference only.

Publishing research using AC004? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

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Secondary Antibodies (17)

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