Positional scanning is an important tool for peptide sequence optimization. This identifies an amino acid of interest at a single position and substitutes it with all other natural amino acids one at a time. Changes in activity identify the preferred amino acid residues at this positions.
Enter your target sequence as well as the position(s) of the amino acid to be substituted, counting from the N-terminus. A total of 20 sequences will be generated for each positional replacement.