Products > Molecular Biology > RNA & DNA Modifying Enzymes > Nucleases

Exonuclease I (E. coli) (RK20531)

Datasheet

Summary

Catalog No.: RK20531

Concentration: 20, 000 U/ml

Background

Exonuclease I (E.coli) Catalyzes the removal of nucleotides from single-stranded DNA in the 3' to 5' direction.
Exonuclease I degrades excess single-stranded primer oligonucleotide from a reaction mixture containing double-stranded extension products.

Product Component

Exonuclease I (E. coli) (20, 000 U/ml)RM20519
10X Exonuclease I Reaction BufferRM20130

Product information

Product SourceAn E.coli strain that carries the cloned Exo I gene from E. coli NM554.
Unit DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 10 nmol of acid-soluble nucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer with 0.17 mg/ml single-stranded [3H]-DNA.
Storage Conditions10 mM Tris-HCl, 100 mM NaCl, 5 mM β-ME, 0.5 mM EDTA, 100 μg/ml BSA, 50% Glycerol, pH 7.5 @ 25°C
Storage Temperature-20°C
Reaction Conditions1X Exonuclease I Reaction Buffer, Incubate at 37°C
Reaction Buffer or Mix1X Exonuclease I Reaction Buffer:
67 mM Glycine-KOH, 6.7 mM MgCl2, 10 mM β-ME, pH 9.5 @ 25°C
Heat Inactivation80°C for 20 min

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