|Product name||Green fluorescent protein / GFP Gene ORF cDNA clone expression plasmid, C-GFPSpark tag|
|NCBI RefSeq||717 bp|
|Type||Expression-Ready ORF Clones|
|RefSeq ORF size||717 bp|
|cDNA description||Green fluorescent protein / GFP Gene ORF cDNA clone expression plasmid, C-GFPSpark tag (AG13105-ACG) - available for purchase from ABclonal - allows for easy subcloning of the ORF (pCMV3-C-GFPSpark Vector) to other plasmid vectors.|
|Restriction site||KpnI + XbaI (6kb + 1.45kb)|
|Tag Ssequence||GFPSpark: GTGAGCAAGGGC……GAGCTGTACAAG|
|Sequence description||A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with AAX31732.1.|
|Sequencing primers||T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)|
|Promoter||Enhanced CMV mammalian cell promoter|
|Antibiotic in E.coli||Kanamycin|
|Antibiotic in mammalian cell||Hygromycin|
|Shipping carrier||Each tube contains lyophilized plasmid.|
|Storage||The lyophilized plasmid can be stored at ambient temperature for three months.|
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