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Cynomolgus Carboxypeptidase A1/CPA1 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag (CG90550-ACR)

Overview

Product nameCynomolgus Carboxypeptidase A1/CPA1 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag
Catalog No.CG90550-ACR
NCBI RefSeq1260 bp
TypeExpression-Ready ORF Clones
Human Carboxypeptidase A1 (CPA1)is secreted as a pancreatic procarboxypeptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group, with the preference of  residues with aromatic or branched aliphatic side chains. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated.

Molecule nameCPA1
RefSeq ORF size1260 bp
cDNA descriptionCynomolgus Carboxypeptidase A1/CPA1 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag (CG90550-ACR) - available for purchase from ABclonal - allows for easy subcloning of the ORF (pCMV3-C-OFPSpark Vector) to other plasmid vectors.
VectorpCMV3-C-OFPSpark
Tag SsequenceOFPSpark Tag: GATAGCACTGAG……CACCTGTTCCAG
Sequencing primersT7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
PromoterEnhanced CMV mammalian cell promoter
Antibiotic in E.coliKanamycin
Antibiotic in mammalian cellHygromycin
Shipping carrierEach tube contains lyophilized plasmid.
StorageThe lyophilized plasmid can be stored at ambient temperature for three months.

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