Product Type > Assay Kits > ELISA Kits > Mouse ELISA Kits

Mouse TNF-alpha ELISA Kit (RK00027)

Publications (120) Datasheet COA

ABclonal:Mouse TNF-alpha ELISA Kit
ABclonal: - Mouse TNF-alpha ELISA Kit (RK00027)

Overview

Product name Mouse TNF-alpha ELISA Kit
Catalog No. RK00027
Size 96 T
Sample 100 ul
Instrument Microplate Reader
Detection Colorimetric
Assay Time 4 hours
Conjugate Biotin
Assay Type Sandwich ELISA
Sample type serum, plasma, cell culture supernates
Cross reacts with Mouse
Product overview The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in Serum, plasma and cell culture supernates
Intended use This Mouse TNF-alpha ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Mouse TNF-alpha in the sample, this Mouse TNF-alpha ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Mouse TNF-alpha concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage instructions Unopened kits can be stored at 2-8°C for 1 year, and opened products must be used within 1 month.
Sensitivity 6.5 pg/mL
Standard curve range 15.6-1000 pg/mL
Components Microtiter Plate
Enzyme Conjugate
Standards
Substrate A
Substrate B
Stop Solution
Wash Solution
Balance Solution
Safety notes 15.6-1000 pg/ml

Documents

Certificate of Compliance

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