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Biotinylated Human IL-2 Monoclonal Antibody, Rabbit MAb(DET) (RM17591)

ABclonal:Western blot - Biotinylated Human IL-2 Monoclonal Antibody,  Rabbit MAb(DET) (RM17591)
ABclonal:Western blot - Biotinylated Human IL-2 Monoclonal Antibody,  Rabbit MAb(DET) (RM17591)
ABclonal:Western blot - Biotinylated Human IL-2 Monoclonal Antibody,  Rabbit MAb(DET) (RM17591)
ABclonal:Western blot - Biotinylated Human IL-2 Monoclonal Antibody,  Rabbit MAb(DET) (RM17591)

Overview

Product name Biotinylated Human IL-2 Monoclonal Antibody, Rabbit MAb(DET)
Catalog No. RM17591
Catagory Elisa Antibody Kit
Application ELISA
Description Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis. IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells.
Immunogen Recombinant Human IL2 Protein
Ala21-Thr153
Accession #P60568
Purirty Affinity purification
Ig Type Rabbit IgG
Endotoxin Level < 1.0 EU/μg of the protein by LAL method.
Formulation Supplied as a 0.2um filtered solution in PBS with 0.1%Braveds MB-1, PH 7.4.
CrossReactivity No cross-reactivity in ELISA with recombinant
rmIL-2, rrIL-2, rhIL-2R, rhIL-1a, rhIL-1b, rhIL-2Ra, rhIL-4, rhIL-7, rhIL-9, rhIL-15
Storage This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.

Avoid repeated freeze-thaw cycles.

Application

Human IL-2 Sanwich ELISA Immunoassay

 Recommended
Concentration
Sample
ELISA Capture2-8ug/mLHuman IL-2 Monoclonal Antibody, Rabbit MAb(CAP)(Cat. No. RM17589)
ELISA Detection0.15-0.5ug/mLBiotinylated Human IL-2 Monoclonal Antibody, Rabbit MAb(DET)(Cat. No. RM17591)
Standard0.78-50pg/mLRecombinant Human IL2 Protein(Cat. No. RP01039)
ABclonal:Western blot - Biotinylated Human IL-2 Monoclonal Antibody,  Rabbit MAb(DET) (RM17591)}

Western blot - Biotinylated Human IL-2 Monoclonal Antibody, Rabbit MAb(DET) (RM17591)

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
ABclonal:Western blot - Biotinylated Human IL-2 Monoclonal Antibody,  Rabbit MAb(DET) (RM17591)}

Western blot - Biotinylated Human IL-2 Monoclonal Antibody, Rabbit MAb(DET) (RM17591)

From the comparison of the standard curve, it is found that ABclonal has high sensitivity.
ABclonal:Western blot - Biotinylated Human IL-2 Monoclonal Antibody,  Rabbit MAb(DET) (RM17591)}

Western blot - Biotinylated Human IL-2 Monoclonal Antibody, Rabbit MAb(DET) (RM17591)

No significant cross-reactivity or interference was observed.
ABclonal:Western blot - Biotinylated Human IL-2 Monoclonal Antibody,  Rabbit MAb(DET) (RM17591)}

Western blot - Biotinylated Human IL-2 Monoclonal Antibody, Rabbit MAb(DET) (RM17591)

Placed at 37°C for 3 days, the stability of the standard curve all conform to CV <10%.

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* For research use only. Not for therapeutic or diagnostic purposes.

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