Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Thymidine Kinase 1 Rabbit pAb (A5612)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Thymidine Kinase 1 Rabbit pAb (A5612)

Western blot analysis of extracts of various cell lines, using Thymidine Kinase 1 antibody (A5612) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Thymidine Kinase 1 Rabbit pAb (A5612)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)

Immunofluorescence analysis of HeLa cells using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)

Immunofluorescence analysis of MCF7 cells using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)

Immunofluorescence analysis of NIH/3T3 cells using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)

Immunofluorescence analysis of PC-12 cells using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name Thymidine Kinase 1 Rabbit pAb
Catalog No. A5612
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 15-194 of human Thymidine Kinase 1 (NP_003249.3).
Sequence SKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFFPDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKAS
Gene ID 7083
Swiss prot P04183
Synonyms TK2; Thymidine Kinase 1
Calculated MW 25kDa
Observed MW 26kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, 293T, Jurkat, Mouse liver, Mouse spleen, Mouse stomach, Rat liver, Rat spleen, Rat stomach
Cellular location Cytoplasm

Documents

Certificate of Compliance

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Lot number

Thymidine Kinase 1 Rabbit pAb images

ABclonal:Western blot - Thymidine Kinase 1 Rabbit pAb (A5612)}

Western blot - Thymidine Kinase 1 Rabbit pAb (A5612)

Western blot analysis of extracts of various cell lines, using Thymidine Kinase 1 antibody (A5612) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Thymidine Kinase 1 Rabbit pAb (A5612)}

Immunohistochemistry - Thymidine Kinase 1 Rabbit pAb (A5612)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)}

Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)

Immunofluorescence analysis of HeLa cells using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)}

Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)

Immunofluorescence analysis of MCF7 cells using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)}

Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)

Immunofluorescence analysis of NIH/3T3 cells using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)}

Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)

Immunofluorescence analysis of PC-12 cells using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on TK1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TK1. (Distance between topics and target gene indicate popularity.) TK1

* Data provided by citexs.com, for reference only.

Publishing research using A5612? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

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Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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