Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

TPI1 Rabbit pAb (A15733)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - TPI1 Rabbit pAb (A15733)

Western blot analysis of various lysates using TPI1 Rabbit pAb (A15733) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - TPI1 Rabbit pAb (A15733)

Immunohistochemistry analysis of TPI1 in paraffin-embedded rat kidney using TPI1 Rabbit pAb (A15733) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TPI1 Rabbit pAb (A15733)

Immunohistochemistry analysis of TPI1 in paraffin-embedded human lung cancer using TPI1 Rabbit pAb (A15733) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TPI1 Rabbit pAb (A15733)

Immunohistochemistry analysis of TPI1 in paraffin-embedded mouse kidney using TPI1 Rabbit pAb (A15733) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - TPI1 Rabbit pAb (A15733)

Immunofluorescence analysis of U-2 OS cells using TPI1 Rabbit pAb (A15733) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name TPI1 Rabbit pAb
Catalog No. A15733
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human TPI1 (NP_000356.1).
Sequence MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ
Gene ID 7167
Swiss prot P60174
Synonyms TIM; TPI; TPID; HEL-S-49; TPI1
Calculated MW 27kDa
Observed MW 30kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:100 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples U-87MG, 293T, DU145, A-431, Mouse stomach, Mouse brain, Mouse heart, Mouse kidnay, Rat brain
Cellular location cytosol, extracellular exosome, extracellular space, nucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

TPI1 Rabbit pAb images

ABclonal:Western blot - TPI1 Rabbit pAb (A15733)}

Western blot - TPI1 Rabbit pAb (A15733)

Western blot analysis of various lysates using TPI1 Rabbit pAb (A15733) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - TPI1 Rabbit pAb (A15733)}

Immunohistochemistry - TPI1 Rabbit pAb (A15733)

Immunohistochemistry analysis of TPI1 in paraffin-embedded rat kidney using TPI1 Rabbit pAb (A15733) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TPI1 Rabbit pAb (A15733)}

Immunohistochemistry - TPI1 Rabbit pAb (A15733)

Immunohistochemistry analysis of TPI1 in paraffin-embedded human lung cancer using TPI1 Rabbit pAb (A15733) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TPI1 Rabbit pAb (A15733)}

Immunohistochemistry - TPI1 Rabbit pAb (A15733)

Immunohistochemistry analysis of TPI1 in paraffin-embedded mouse kidney using TPI1 Rabbit pAb (A15733) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - TPI1 Rabbit pAb (A15733)}

Immunofluorescence - TPI1 Rabbit pAb (A15733)

Immunofluorescence analysis of U-2 OS cells using TPI1 Rabbit pAb (A15733) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on TPI1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TPI1. (Distance between topics and target gene indicate popularity.) TPI1

* Data provided by citexs.com, for reference only.

Publishing research using A15733? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Proteins (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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