Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

THOP1 Rabbit pAb (A8756)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - THOP1 Rabbit pAb (A8756)

Western blot analysis of extracts of various cell lines, using THOP1 antibody (A8756) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Overview

Product name THOP1 Rabbit pAb
Catalog No. A8756
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a kininase that uses zinc as a cofactor. The encoded oligopeptidase cleaves cytosolic peptides, making them unavailable for display on antigen-presenting cells. This protein also cleaves neuropeptides under 20 aa in length and can degrade beta-amyloid precursor protein to amyloidogenic peptides.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human THOP1 (NP_003240.1).
Sequence MKPPAACAGDMADAASPCSVVNDLRWDLSAQQIEERTRELIEQTKRVYDQVGTQEFEDVSYESTLKALADVEVTYTVQRNILDFPQHVSPSKDIRTASTEADKKLSEFDVEMSMREDVYQRIVWLQEKVQKDSLRPEAARYLERLIKLGRRNGLHLPRETQENIKRIKKKLSLLCIDFNKNLNEDTTFLPFTLQELGGLPEDFLNSLEKMEDGKLKVTLKYPHYFPLLKKCHVPETRRKVEEAFNCRCKEENCAILKELV
Gene ID 7064
Swiss prot P52888
Synonyms TOP; MP78; EP24.15; MEPD_HUMAN; THOP1
Calculated MW 79kDa
Observed MW 79kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse skeletal muscle, Mouse heart, Mouse lung, Rat liver, Rat kidney
Cellular location Cytoplasm
Customer validation

WB (Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

THOP1 Rabbit pAb images

ABclonal:Western blot - THOP1 Rabbit pAb (A8756)}

Western blot - THOP1 Rabbit pAb (A8756)

Western blot analysis of extracts of various cell lines, using THOP1 antibody (A8756) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Related Targets

Based on existing publications, the following genes are closely related to research on THOP1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to THOP1. (Distance between topics and target gene indicate popularity.) THOP1

* Data provided by citexs.com, for reference only.

Publishing research using A8756? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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