Product Type > Proteins

Recombinant SARS-CoV-2 Spike RBD Protein (RP01258)

Publications (7) Datasheet COA

ABclonal:Recombinant SARS-CoV-2 Spike RBD  Protein

Overview

Product name Recombinant SARS-CoV-2 Spike RBD Protein
Catalog No. RP01258
Description Recombinant SARS-CoV-2(2019-nCoV) Spike RBD-His Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of SARS-COV-2(2019-nCoV) Spike RBD-His (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.
Bio-Activity 1.Measured by its binding ability in a functional ELISA.Immobilized SARS-CoV-2 Spike RBD (Catalog: RP01258) at 2 μg/mL (100 μL/well) can bind Human ACE-2 (Catalog: RP01275) with a linear range of 0.001-2.96 ng/mL.
2.Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 35.3 nM as determined in a SPR assay (Nicoya OpenSPR).
3.Measured by its binding ability in a functional ELISA.Immobilized SARS-CoV-2 Spike RBD (Catalog: RP01258) at 2 μg/mL (100 μL/well) can bind Human ACE-2 (Catalog: RP01275) with a linear range of 0.001-1.69 ng/mL.
Purity >95% by SDS-PAGE;> 95% by HPLC
Endotoxin < 0.1 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation.
Species SARS-CoV-2
Calculated MW 25.9kDa
Synonym Envelope; SARS-CoV-2 Spike RBD (N501Y); Spike; Spike ECD; Spike RBD; Spike S1; Spike S2; Spike S2 ECD; S1-RBD protein; NCP-CoV RBD Protein; novel coronavirus RBD Protein; 2019-nCoV RBD Protein; S glycoprotein Subunit1 RBD Protein
Expressed Host HEK293 cells
Tag C-His
Gene ID 43740568
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
or This product is stable at ≤ -70°C for up to 6 months from the date of receipt.
For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Inquire About This Product

Submit your question about RP01258 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using RP01258? Please let us know so that we can cite the reference in this datasheet.

Proteins (19)

Antibodies (12)

ELISA Kits (1)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Request a free sample
Contact us to order