Publications (7) Datasheet COA
Product name | Recombinant SARS-CoV-2 Spike RBD Protein |
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Catalog No. | RP01258 |
Description | Recombinant SARS-CoV-2(2019-nCoV) Spike RBD-His Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of SARS-COV-2(2019-nCoV) Spike RBD-His (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus. |
Bio-Activity |
1.Measured by its binding ability in a functional ELISA.Immobilized SARS-CoV-2 Spike RBD (Catalog: RP01258) at 2 μg/mL (100 μL/well) can bind Human ACE-2 (Catalog: RP01275) with a linear range of 0.001-2.96 ng/mL. 2.Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 35.3 nM as determined in a SPR assay (Nicoya OpenSPR). 3.Measured by its binding ability in a functional ELISA.Immobilized SARS-CoV-2 Spike RBD (Catalog: RP01258) at 2 μg/mL (100 μL/well) can bind Human ACE-2 (Catalog: RP01275) with a linear range of 0.001-1.69 ng/mL. |
Purity | >95% by SDS-PAGE;> 95% by HPLC |
Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation. |
Species | SARS-CoV-2 |
Calculated MW | 25.9kDa |
Synonym | Envelope; SARS-CoV-2 Spike RBD (N501Y); Spike; Spike ECD; Spike RBD; Spike S1; Spike S2; Spike S2 ECD; S1-RBD protein; NCP-CoV RBD Protein; novel coronavirus RBD Protein; 2019-nCoV RBD Protein; S glycoprotein Subunit1 RBD Protein |
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Expressed Host | HEK293 cells |
Tag | C-His |
Gene ID | 43740568 |
Storage | Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. or This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.