Product name | Recombinant MERS-CoV Spike S1 Protein |
---|---|
Catalog No. | RP01303 |
Description | Recombinant MERS-CoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Tyr18-Glu725) of mers-cov Spike S1 (Accession #YP_009047204.1) fused with a mFc tag at the C-terminus. |
Bio-Activity |
Measured by its binding ability in a functional ELISA. Immobilized Human CD26 (Catalog: RP01300LQ) at 2μg/mL (100μL/well) can bind MERS-CoV Spike S1 with a linear range of 0.5-420.21ng/mL. |
Purity | > 95% by SDS-PAGE. |
Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
Species | MERS-CoV |
Storage | Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. |
---|---|
Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
Submit your question about RP01303 below and we will get back to you within one business day.
Alternatively, call us at 888.754.5670.
Publishing research using RP01303? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.