Product name | Active Recombinant Human Latent TGF-beta 1(C33S) Protein |
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Catalog No. | RP00161 |
Description | Active Recombinant Human Latent TGF-beta 1(C33S) Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Leu30-Ser390(C33S)) of human TGF-beta 1 (Accession #NP_000651.3) fused with an 8×His tag at the N-terminus. |
Bio-Activity |
1.Measured by its ability to inhibit cell proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-2 ng/mL, corresponding to a specific activity of 5.0×105~2.0×106 units/mg. 2.Measured by its binding ability in a functional ELISA. Immobilized recombinant human TGFB1 at 2 μg/mL (100 μL/well) can bind recombinant human TGFBR2 with a linear range of 1-5 ng/mL. 3.Measured by its ability to inhibit the IL-4(Catalog: RP01161)-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 9.75-39 ng/mL, corresponding to a specific activity of 2.6×104~1.0×105 units/mg. |
Purity | > 95% by SDS-PAGE. |
Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
Species | Human |
Storage | Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile 4 mM HCl. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.