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Recombinant Human TGF-beta 1 Protein (RP00161)

Publication (1) Datasheet

Overview

Product nameRecombinant Human TGF-beta 1 Protein
Catalog No.RP00161
DescriptionRecombinant Human TGF-beta 1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Leu30-Ser390(C33S)) of human TGF-beta 1 (Accession #NP_000651.3) fused with an 8×His tag at the N-terminus.
Bio-ActivityMeasured by its ability to inhibit cell proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-2 ng/mL.|Measured by its binding ability in a functional ELISA. Immobilized recombinant human TGFB1 at 2 ug/mL (100 µL/well) can bind recombinant human TGFBR2 with a linear range of 1-5 ng/mL.
Purity> 95% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
SpeciesHuman
SynonymCED; DPD1; LAP; TGF-beta 1; TGFB; TGFbeta
Expressed HostHEK293 cells
TagN-8×His
Swiss-ProP01137
Gene ID7040
StorageStore the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
ReconstitutionReconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

* For research use only. Not for therapeutic or diagnostic purposes.

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