Product Type > Proteins

Recombinant Human Semaphorin-4D/SEMA4D/CD100 Protein (RP00981)

Datasheet

Overview

Product name Recombinant Human Semaphorin-4D/SEMA4D/CD100 Protein
Catalog No. RP00981
Description Recombinant Human Semaphorin-4D/SEMA4D/CD100 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met22-Arg734) of human Semaphorin 4D (Accession #NP_006369.3) fused with a 6×His tag at the C-terminus.
Bio-Activity Measured by its binding ability in a functional ELISA. Immobilized Human SEMA4D Protein at 1μg/mL (100 μL/well) can bind CD100 Rabbit mAb with a linear range of 0.012-0.88 ng/mL.
Purity > 97% by SDS-PAGE.
Endotoxin < 0.1 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species Human
This protein is a member of the semaphorin family of proteins and an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. SEMA4D was the first semaphorin described to have immune functions and serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Proteolytic cleavage of SEMA4D from the cell surface gives rise to a soluble fragment of SEMA4D (sSEMA4D). Similar to the transmembranal form, sSEMA4D is thought to have immunoregulatory properties.
Synonym SEMA4D; C9orf164; CD100; M-sema-G; SEMAJ; coll-4; Semaphorin 4D / CD100
Expressed Host HEK293 cells
Tag C-His
Swiss-Prot Q92854
Gene ID 10507
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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