Product Type > Proteins

Recombinant Human Semaphorin-3G/SEMA3G Protein (RP01400)

Datasheet

Overview

Product name Recombinant Human Semaphorin-3G/SEMA3G Protein
Catalog No. RP01400
Description Recombinant Human Semaphorin-3G/SEMA3G Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Thr782) of human SEMA3G (Accession #NP_064548.1) fused with a 6×His tag at the C-terminus.
Purity > 90% by SDS-PAGE.
Endotoxin Please contact us for more information.
Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Species Human
Semaphorin 3G (Sema3G), also known as Semaphorin sem2, is a class 3 member of the semaphorin family initially identified as regulators of neuron-axonal guidance. More than 30 semaphorins have been discovered and are divided into eight classes: classes 1 and 4-7 are membrane-associated, while classes 2, 3, and 8 are in a secreted form. In vitro analysis shows Sema 3G binds to Neuropilin-2, which forms a receptor complex with Plexin-D1. Via this pathways Sema 3G controls lymphatic vascular patterning by the means of cellular collapse. It shows protective effects in ischemic retinopathies by coordinating interactions of beta -catenin and VE-Cadherin in endothelium. Sema 3G has been shown to be involved in adipocyte differentiation. Knockdown of Sema3G inhibited weight gain through PI3K/Akt/GSK3 beta signaling in the adipose tissue and the AMPK/SREBP-1c pathway in the liver. Thus, Sema 3G is an adipokine essential for adipogenesis, lipogenesis, and insulin resistance and is associated with obesity.
Synonym SEMA3G; sem2
Expressed Host HEK293 cells
Tag C-His
Swiss-Prot Q9NS98
Gene ID 56920
Storage Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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