Product Type > Proteins

Recombinant Human IgG1 Protein (RP01354)

Publication (1) Datasheet

Overview

Product name Recombinant Human IgG1 Protein
Catalog No. RP01354
Bio-Activity Measured by its binding ability in a functional ELISA. Immobilized Human Fc-gamma RII-a(CD32a) at 1 μg/mL (100 μL/well) can bind IgG1 Fc with a linear range of 0.156-3.47 μg/mL.
Purity > 95% by SDS-PAGE.
Endotoxin <0.1EU/ug
Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Species Human
As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (5 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases.
Synonym Human IgG; IGHG1; COB1; YAP; YAP2; YAP65; YKI; YAP1; human IgG (Fc)
Expressed Host HEK293 cells
Tag C-His
Swiss-Prot P01857
Gene ID 3500
Storage Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Inquire About This Product

Submit your question about RP01354 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using RP01354? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

!OUT OF STOCK

See Below for Alternatives
Alternative Products
Contact us to order