Product name | Recombinant Human IL-4 Protein |
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Catalog No. | RP01597 |
Description | Recombinant Human IL-4 Protein is produced by Pichia expression system. The target protein is expressed with sequence (His25-Ser153) of human IL-4 (Accession #NP_000580.1) fused with and a 6×His tag at the N-terminus. |
Bio-Activity |
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.25-1.00 ng/mL. |
Purity | > 95% by SDS-PAGE. |
Endotoxin | <0.1EU/μg |
Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
Species | Human |
Storage | Store the lyophilized protein at -20°C to -80°C for 12 months. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.