Product Type > Proteins

Recombinant Human Complement C5A Protein (RP01128)

Datasheet

Overview

Product name Recombinant Human Complement C5A Protein
Catalog No. RP01128
Description Recombinant Human Complement C5 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Leu679-Arg751) of human C5a (Accession #NP_001726.2).
Bio-Activity Measured by its ability to induce N-acetyl-β-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. The ED50 for this effect is typically 5-15 ng/mL.
Purity > 94 % by SDS-PAGE.
Endotoxin Please contact us for more information.
Formulation Lyophilized from a 0.22 μm filtered solution PBS, pH 7.4.Contact us for customized product form or formulation.
Species Human
This protein is a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the C5 alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mutations in this gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections. Alternative splicing results in multiple transcript variants.
Synonym C5; C5D; C5a; C5b; CPAMD4; ECLZB
Expressed Host E. coli
Tag No tag
Swiss-Prot P01031
Gene ID 727
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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Antibodies (1)

* For research use only. Not for therapeutic or diagnostic purposes.

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