Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-PERK-T982 Rabbit pAb (AP0886)

Publications (30) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-PERK-T982 Rabbit pAb (AP0886)

Western blot analysis of lysates from 293T cells, using Phospho-PERK-T982 Rabbit pAb (AP0886) at 1:1000 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Phospho-PERK-T982 Rabbit pAb (AP0886)

Immunohistochemistry analysis of Phospho-PERK-T982 in paraffin-embedded human esophagus tissue using Phospho-PERK-T982 Rabbit pAb (AP0886) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-PERK-T982 Rabbit pAb (AP0886)

Immunohistochemistry analysis of Phospho-PERK-T982 in paraffin-embedded rat lung tissue using Phospho-PERK-T982 Rabbit pAb (AP0886) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-PERK-T982 Rabbit pAb (AP0886)

Immunohistochemistry analysis of Phospho-PERK-T982 in paraffin-embedded mouse Intestine tissue using Phospho-PERK-T982 Rabbit pAb (AP0886) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-PERK-T982 Rabbit pAb (AP0886)

Immunohistochemistry analysis of Phospho-PERK-T982 in paraffin-embedded mouse liver tissue using Phospho-PERK-T982 Rabbit pAb (AP0886) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

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Overview

Product name Phospho-PERK-T982 Rabbit pAb
Catalog No. AP0886
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T982 of human PERK (NP_004827.4).
Sequence RHTGQ
Gene ID 9451
Swiss prot Q9NZJ5
Synonyms PEK; WRS; PERK; Phospho-PERK-T982
Calculated MW 125kDa
Observed MW 170kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples 293T, 293T+IGF-1
Cellular location Endoplasmic reticulum membrane, Single-pass type I membrane protein
Customer validation

WB (Mus musculus, Homo sapiens, Rattus norvegicus, Chlorocebus aethiops, Danio rerio, Homo sapiensCircetidae)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-PERK-T982 Rabbit pAb images

ABclonal:Western blot - Phospho-PERK-T982 Rabbit pAb (AP0886)}

Western blot - Phospho-PERK-T982 Rabbit pAb (AP0886)

Western blot analysis of lysates from 293T cells, using Phospho-PERK-T982 Rabbit pAb (AP0886) at 1:1000 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Phospho-PERK-T982 Rabbit pAb (AP0886)}

Immunohistochemistry - Phospho-PERK-T982 Rabbit pAb (AP0886)

Immunohistochemistry analysis of Phospho-PERK-T982 in paraffin-embedded human esophagus tissue using Phospho-PERK-T982 Rabbit pAb (AP0886) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-PERK-T982 Rabbit pAb (AP0886)}

Immunohistochemistry - Phospho-PERK-T982 Rabbit pAb (AP0886)

Immunohistochemistry analysis of Phospho-PERK-T982 in paraffin-embedded rat lung tissue using Phospho-PERK-T982 Rabbit pAb (AP0886) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-PERK-T982 Rabbit pAb (AP0886)}

Immunohistochemistry - Phospho-PERK-T982 Rabbit pAb (AP0886)

Immunohistochemistry analysis of Phospho-PERK-T982 in paraffin-embedded mouse Intestine tissue using Phospho-PERK-T982 Rabbit pAb (AP0886) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-PERK-T982 Rabbit pAb (AP0886)}

Immunohistochemistry - Phospho-PERK-T982 Rabbit pAb (AP0886)

Immunohistochemistry analysis of Phospho-PERK-T982 in paraffin-embedded mouse liver tissue using Phospho-PERK-T982 Rabbit pAb (AP0886) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on EIF2AK3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to EIF2AK3. (Distance between topics and target gene indicate popularity.) EIF2AK3

* Data provided by citexs.com, for reference only.

Publishing research using AP0886? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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