Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb (AP1049)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Mouse, Rat

ABclonal:Western blot - Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb (AP1049)

Western blot analysis of extracts of various cell lines, using Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb (AP1049) at 1:1000 dilution.Both NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Overview

Product name Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb
Catalog No. AP1049
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC1596

Background

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Immunogen information

Immunogen A synthetic phosphorylated peptide around S474 of human PAK4PAK4 (O96013).
Sequence RKSLV
Gene ID 102985714456924
Swiss prot O96013(PAK4-Antigen), Q9P286(PAK5), Q9NQU5(PAK6)
Synonyms PAK4; KIAA1142; PAK5KIAA1264; PAK6; Phospho-PAK4/PAK5/PAK6-S474/S560/S602
Calculated MW 64, 80, 75 kDa
Observed MW 64kDa, 75kDa, 70kDa

Applications

Reactivity Mouse, Rat
Tested applications Testing results
WB Mouse
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples NIH/3T3 PMA, C6 PMA
Cellular location Cytoplasm, Nucleus

Documents

Certificate of Compliance

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Lot number

Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb images

ABclonal:Western blot - Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb (AP1049)}

Western blot - Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb (AP1049)

Western blot analysis of extracts of various cell lines, using Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb (AP1049) at 1:1000 dilution.Both NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PAK4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PAK4. (Distance between topics and target gene indicate popularity.) PAK4

* Data provided by citexs.com, for reference only.

Publishing research using AP1049? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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