Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PTK6 Rabbit pAb (A7497)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - PTK6 Rabbit pAb (A7497)

Western blot analysis of various lysates using PTK6 Rabbit pAb (A7497) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - PTK6 Rabbit pAb (A7497)

Immunofluorescence analysis of A549 cells using PTK6 Rabbit pAb (A7497). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name PTK6 Rabbit pAb
Catalog No. A7497
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 222-451 of human PTK6 (NP_005966.1).
Sequence SRDNLLHQQMLQSEIQAMKKLRHKHILALYAVVSVGDPVYIITELMAKGSLLELLRDSDEKVLPVSELLDIAWQVAEGMCYLESQNYIHRDLAARNILVGENTLCKVGDFGLARLIKEDVYLSHDHNIPYKWTAPEALSRGHYSTKSDVWSFGILLHEMFSRGQVPYPGMSNHEAFLRVDAGYRMPCPLECPPSVHKLMLTCWCRDPEQRPCFKALRERLSSFTSYENPT
Gene ID 5753
Swiss prot Q13882
Synonyms BRK; PTK6
Calculated MW 52kDa
Observed MW 60kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples SW480, BT-474, MCF7, A-549, NIH/3T3, Mouse liver, Mouse kidney, Mouse testis, Rat kidney
Cellular location Cell projection, Cytoplasm, Membrane, Nucleus, ruffle

Documents

Certificate of Compliance

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Research Area

PTK6 Rabbit pAb images

ABclonal:Western blot - PTK6 Rabbit pAb (A7497)}

Western blot - PTK6 Rabbit pAb (A7497)

Western blot analysis of various lysates using PTK6 Rabbit pAb (A7497) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - PTK6 Rabbit pAb (A7497)}

Immunofluorescence - PTK6 Rabbit pAb (A7497)

Immunofluorescence analysis of A549 cells using PTK6 Rabbit pAb (A7497). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on PTK6. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to PTK6. (Distance between topics and target gene indicate popularity.) PTK6

* Data provided by citexs.com, for reference only.

Publishing research using A7497? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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