Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PPP4C Rabbit pAb (A2109)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - PPP4C Rabbit pAb (A2109)

Western blot analysis of various lysates using PPP4C Rabbit pAb (A2109) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - PPP4C Rabbit pAb (A2109)

Immunohistochemistry analysis of PPP4C in paraffin-embedded rat liver using PPP4C Rabbit pAb (A2109) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PPP4C Rabbit pAb (A2109)

Immunohistochemistry analysis of PPP4C in paraffin-embedded human lung cancer using PPP4C Rabbit pAb (A2109) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PPP4C Rabbit pAb (A2109)

Immunohistochemistry analysis of PPP4C in paraffin-embedded human colon using PPP4C Rabbit pAb (A2109) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunoprecipitation - PPP4C Rabbit pAb (A2109)

Immunoprecipitation analysis of 200 μg extracts of 293T cells using 1 μg PPP4C antibody (A2109). Western blot was performed from the immunoprecipitate using PPP4C antibody (A2109) at a dilution of 1:1000.

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Overview

Product name PPP4C Rabbit pAb
Catalog No. A2109
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Enables protein serine/threonine phosphatase activity. Involved in regulation of double-strand break repair via homologous recombination. Located in cytosol; nucleoplasm; and plasma membrane.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human PPP4C (NP_002711.1).
Sequence MAEISDLDRQIEQLRRCELIKESEVKALCAKAREILVEESNVQRVDSPVTVCGDIHGQFYDLKELFRVGGDVPETNYLFMGDFVDRGFYSVETFLLLLALKVRYPDRITLIRGNHESRQITQVYGFYDECLRKYGSVTVWRYCTEIFDYLSLSAIIDGKIFCVHGGLSPSIQTLDQIRTIDRKQEVPHDGPMCDLLWSDPEDTTGWGVSPRGAGYLFGSDVVAQFNAANDIDMICRAHQLVMEGYKWHFNETVLTVWSAPNYCYRCGNVAAILELDEHLQKDFIIFEAAPQETRGIPSKKPVADYFL
Gene ID 5531
Swiss prot P60510
Synonyms PP4; PPX; PP-X; PP4C; PPH3; PPP4; PPP4C
Calculated MW 35kDa
Observed MW 36kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunoprecipitation    
Positive samples 293T, NIH/3T3, MCF-7, HO-8910PM, Mouse testis, Mouse spleen
Cellular location Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center

Documents

Certificate of Compliance

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Lot number

Research Area

PPP4C Rabbit pAb images

ABclonal:Western blot - PPP4C Rabbit pAb (A2109)}

Western blot - PPP4C Rabbit pAb (A2109)

Western blot analysis of various lysates using PPP4C Rabbit pAb (A2109) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - PPP4C Rabbit pAb (A2109)}

Immunohistochemistry - PPP4C Rabbit pAb (A2109)

Immunohistochemistry analysis of PPP4C in paraffin-embedded rat liver using PPP4C Rabbit pAb (A2109) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PPP4C Rabbit pAb (A2109)}

Immunohistochemistry - PPP4C Rabbit pAb (A2109)

Immunohistochemistry analysis of PPP4C in paraffin-embedded human lung cancer using PPP4C Rabbit pAb (A2109) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PPP4C Rabbit pAb (A2109)}

Immunohistochemistry - PPP4C Rabbit pAb (A2109)

Immunohistochemistry analysis of PPP4C in paraffin-embedded human colon using PPP4C Rabbit pAb (A2109) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunoprecipitation - PPP4C Rabbit pAb (A2109)}

Immunoprecipitation - PPP4C Rabbit pAb (A2109)

Immunoprecipitation analysis of 200 μg extracts of 293T cells using 1 μg PPP4C antibody (A2109). Western blot was performed from the immunoprecipitate using PPP4C antibody (A2109) at a dilution of 1:1000.

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Related Targets

Based on existing publications, the following genes are closely related to research on PPP4C. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PPP4C. (Distance between topics and target gene indicate popularity.) PPP4C

* Data provided by citexs.com, for reference only.

Publishing research using A2109? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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