Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PLD4 Rabbit pAb (A15207)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Rat

ABclonal:Western blot - PLD4 Rabbit pAb (A15207)

Western blot analysis of various lysates using PLD4 Rabbit pAb (A15207) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

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Overview

Product name PLD4 Rabbit pAb
Catalog No. A15207
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Predicted to enable single-stranded DNA 5'-3' exodeoxyribonuclease activity. Predicted to be involved in hematopoietic progenitor cell differentiation; phagocytosis; and regulation of cytokine production involved in inflammatory response. Predicted to be located in early endosome and endoplasmic reticulum membrane. Predicted to be active in several cellular components, including endoplasmic reticulum; phagocytic vesicle; and trans-Golgi network membrane.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 52-200 of human PLD4 (NP_620145.2).
Sequence WQVPRPPTWGQVQPKDVPRSWEHGSSPAWEPLEAEARQQRDSCQLVLVESIPQDLPSAAGSPSAQPLGQAWLQLLDTAQESVHVASYYWSLTGPDIGVNDSSSQLGEALLQKLQQLLGRNISLAVATSSPTLARTSTDLQVLAARGAHV
Gene ID 122618
Swiss prot Q96BZ4
Synonyms C14orf175; PLD4
Calculated MW 56kDa
Observed MW 64kDa

Applications

Reactivity Human, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples K-562, Rat thymus, Rat lung, Rat spleen
Cellular location Membrane, Single-pass membrane protein

Documents

Certificate of Compliance

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Lot number

Research Area

PLD4 Rabbit pAb images

ABclonal:Western blot - PLD4 Rabbit pAb (A15207)}

Western blot - PLD4 Rabbit pAb (A15207)

Western blot analysis of various lysates using PLD4 Rabbit pAb (A15207) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PLD4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PLD4. (Distance between topics and target gene indicate popularity.) PLD4

* Data provided by citexs.com, for reference only.

Publishing research using A15207? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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