Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PCM1 Rabbit pAb (A5696)

Publications (5) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - PCM1 Rabbit pAb (A5696)

Western blot analysis of lysates from Jurkat cells, using PCM1 Rabbit pAb (A5696) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - PCM1 Rabbit pAb (A5696)

Immunohistochemistry analysis of PCM1 in paraffin-embedded mouse liver using PCM1 Rabbit pAb (A5696) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PCM1 Rabbit pAb (A5696)

Immunohistochemistry analysis of PCM1 in paraffin-embedded human liver cancer using PCM1 Rabbit pAb (A5696) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PCM1 Rabbit pAb (A5696)

Immunofluorescence analysis of NIH/3T3 cells using PCM1 Rabbit pAb (A5696) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PCM1 Rabbit pAb (A5696)

Immunofluorescence analysis of U2OS cells using PCM1 Rabbit pAb (A5696) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name PCM1 Rabbit pAb
Catalog No. A5696
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a component of centriolar satellites, which are electron dense granules scattered around centrosomes. Inhibition studies show that this protein is essential for the correct localization of several centrosomal proteins, and for anchoring microtubules to the centrosome. Chromosomal aberrations involving this gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PCM1 (NP_006188.4).
Sequence MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKI
Gene ID 5108
Swiss prot Q15154
Synonyms PTC4; RET/PCM-1; PCM1
Calculated MW 229kDa
Observed MW 270kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples Jurkat
Cellular location Cytoplasm, Cytoplasmic granule, centriolar satellite, centrosome, cilium basal body, cytoskeleton, microtubule organizing center
Customer validation

IF (Rattus norvegicus, Homo sapiens)

WB (Rattus norvegicus, Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

PCM1 Rabbit pAb images

ABclonal:Western blot - PCM1 Rabbit pAb (A5696)}

Western blot - PCM1 Rabbit pAb (A5696)

Western blot analysis of lysates from Jurkat cells, using PCM1 Rabbit pAb (A5696) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - PCM1 Rabbit pAb (A5696)}

Immunohistochemistry - PCM1 Rabbit pAb (A5696)

Immunohistochemistry analysis of PCM1 in paraffin-embedded mouse liver using PCM1 Rabbit pAb (A5696) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PCM1 Rabbit pAb (A5696)}

Immunohistochemistry - PCM1 Rabbit pAb (A5696)

Immunohistochemistry analysis of PCM1 in paraffin-embedded human liver cancer using PCM1 Rabbit pAb (A5696) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - PCM1 Rabbit pAb (A5696)}

Immunofluorescence - PCM1 Rabbit pAb (A5696)

Immunofluorescence analysis of NIH/3T3 cells using PCM1 Rabbit pAb (A5696) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PCM1 Rabbit pAb (A5696)}

Immunofluorescence - PCM1 Rabbit pAb (A5696)

Immunofluorescence analysis of U2OS cells using PCM1 Rabbit pAb (A5696) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on PCM1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PCM1. (Distance between topics and target gene indicate popularity.) PCM1

* Data provided by citexs.com, for reference only.

Publishing research using A5696? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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