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Human VEGFR2 / Flk-1 / CD309 / KDR Protein (Fc Tag) (10012-H02H)

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Overview

Product nameHuman VEGFR2 / Flk-1 / CD309 / KDR Protein (Fc Tag)
Catalog No.10012-H02H
SpeciesHuman
SynonymCD309, Flk-1, FLK1, VEGFR, VEGFR2
Protein ConstructionHigh quality, high purity and low endotoxin recombinant Human VEGFR2 / Flk-1 / CD309 / KDR Protein (Fc Tag) (10012-H02H), tested reactivity in Human and has been validated in SDS-PAGE.100% guaranteed.
Expressed HostHuman Cells
TagC-human IgG1-Fc
Purity> 97 % as determined by SDS-PAGE
Bio-Activity1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human VEGFA at 1.25 μg/ml (100 μl/well) can bind VEGFR2 with a linear range of 1.25-40.0 ng/ml. 2. Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 10-40 ng/mL in the presence of 10 ng/mL recombinant human VEGF165.
Endotoxin< 1.0 EU per μg of the protein as determined by the LAL method
StabilitySamples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminalAla 20
Molecule massThe recombinant human VEGFR2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 979 amino acids and predicts a molecular mass of 109 kDa. The apparent molecular mass of rh VEGFR2/Fc monomer is approximatly 150-160 in SDS-PAGE under reducing conditions.
ShippingIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
StorageStore it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
ReconstitutionA hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

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