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Human ICAM-1 / CD54 Protein (His & Fc Tag) (10346-H03H)

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Overview

Product nameHuman ICAM-1 / CD54 Protein (His & Fc Tag)
Catalog No.10346-H03H
SpeciesHuman
SynonymBB2, CD54, ICAM-1, P3.58
Protein ConstructionHigh quality, high purity and low endotoxin recombinant Human ICAM-1 / CD54 Protein (His & Fc Tag) (10346-H03H), tested reactivity in Human and has been validated in SDS-PAGE.100% guaranteed.
Expressed HostHuman Cells
TagC-human IgG1-Fc & His
Purity> 95 % as determined by SDS-PAGE
Bio-ActivityMeasured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 10E4 cells/well are added to Recombinant Human ICAM-1/CD54 coated plates (12.5 μg/mL with 100 μL/well), >40% cells will adhere after 1 hour of incubation at 37℃.
Endotoxin< 1.0 EU per μg of the protein as determined by the LAL method
StabilitySamples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminalGln 28
Molecule massThe recombinant human ICAM1/Fc is a disulfide-linked homodimer. The reduced monomer consists of 700 amino acids and has a predicted molecular mass of 77.4 kDa. As a result of glycosylation, the apparent molecular mass of rh ICAM1/Fc monomer migrates with an apparent molecular mass of 100-110 kDa in SDS-PAGE under reducing conditions.
ShippingIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
StorageStore it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
ReconstitutionA hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

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