|Product name||Human CD50 / ICAM-3 Protein (His & Fc Tag)|
|Synonym||CD50, CDW50, ICAM-R|
|Protein Construction||High quality, high purity and low endotoxin recombinant Human CD50 / ICAM-3 Protein (His & Fc Tag) (10333-H03H), tested reactivity in Human and has been validated in SDS-PAGE.100% guaranteed.|
|Expressed Host||Human Cells|
|Tag||C-human IgG1-Fc & His|
|Purity||> 97 % as determined by SDS-PAGE|
|Bio-Activity||Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM3-coated plates (12.5 μg/ml, 100 μl/well), approximately 45%-60% will adhere specifically .|
|Endotoxin||< 1.0 EU per μg of the protein as determined by the LAL method|
|Stability||Samples are stable for up to twelve months from date of receipt at -70℃|
|Predicted N terminal||Gln 30|
|Molecule mass||The recombinant human ICAM3/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 703 amino acids and predicts a molecular mass of 77.2 kDa. By SDS-PAGE, the apparent molecular mass of rh ICAM3/Fc is approximately 125-135 kDa due to the glycosylation.|
|Shipping||In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|Storage||Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
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