|Product name||Human CCNE1 / Cyclin-E1 Protein (His & GST Tag)|
|Protein Construction||High quality, high purity and low endotoxin recombinant Human CCNE1 / Cyclin-E1 Protein (His & GST Tag) (10902-H20B), tested reactivity in Human and has been validated in SDS-PAGE.100% guaranteed.|
|Expressed Host||Baculovirus-Insect Cells|
|Tag||N-GST & His|
|Purity||> 82 % as determined by SDS-PAGE|
|Bio-Activity||Measured by its binding ability in a functional ELISA. Immobilized human HG3C-CCNE1 (Cat:10902-H20B) at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CDK4 (Cat:10732-H09B), The EC50 of biotinylated human GST-CDK4 (Cat:10732-H09B) is 45.0-106.0 ng/ml.|
|Endotoxin||< 1.0 EU per μg of the protein as determined by the LAL method|
|Stability||Samples are stable for up to twelve months from date of receipt at -70℃|
|Predicted N terminal||Met|
|Molecule mass||The recombinant human CCNE1/GST chimera consists of 647 amino acids and has a calculated molecular mass of 75 KDa. It migrates as an approximately 70 KDa band in SDS-PAGE under reducing conditions.|
|Shipping||In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|Storage||Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
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