Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CWC22 Rabbit pAb (A13116)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Mouse

ABclonal:Western blot - CWC22 Rabbit pAb (A13116)

Western blot analysis of various lysates using CWC22 Rabbit pAb (A13116) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Overview

Product name CWC22 Rabbit pAb
Catalog No. A13116
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Enables RNA binding activity. Involved in mRNA splicing, via spliceosome. Located in cytosol and nuclear speck. Part of U2-type catalytic step 1 spliceosome; U2-type catalytic step 2 spliceosome; and U2-type precatalytic spliceosome.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 639-908 of human CWC22 (NP_065994.1).
Sequence LTDELREHLKNTPKVIVAQKPDVEQNKSSPSSSSSASSSSESDSSDSDSDSSDSSSESSSEESDSSSISSHSSASANDVRKKGHGKTRSKEVDKLIRNQQTNDRKQKERRQEHGHQETRTERERRSEKHRDQNSSGSNWRDPITKYTSDKDVPSERNNYSRVANDRDQEMHIDLENKHGDPKKKRGERRNSFSENEKHTHRIKDSENFRRKDRSKSKEMNRKHSGSRSDEDRYQNGAERRWEKSSRYSEQSRESKKNQDRRREKSPAKQK
Gene ID 57703
Swiss prot Q9HCG8
Synonyms NCM; fSAPb; EIF4GL; CWC22
Calculated MW 105kDa
Observed MW 110kDa

Applications

Reactivity Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse liver, Mouse testis
Cellular location Nucleus, Nucleus speckle

Documents

Certificate of Compliance

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Lot number

CWC22 Rabbit pAb images

ABclonal:Western blot - CWC22 Rabbit pAb (A13116)}

Western blot - CWC22 Rabbit pAb (A13116)

Western blot analysis of various lysates using CWC22 Rabbit pAb (A13116) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Related Targets

Based on existing publications, the following genes are closely related to research on CWC22. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CWC22. (Distance between topics and target gene indicate popularity.) CWC22

* Data provided by citexs.com, for reference only.

Publishing research using A13116? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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