Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CHRAC1 Rabbit pAb (A7372)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - CHRAC1 Rabbit pAb (A7372)

Western blot analysis of lysates from A549 cells using CHRAC1 Rabbit pAb(A7372) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

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Overview

Product name CHRAC1 Rabbit pAb
Catalog No. A7372
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

CHRAC1 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-131 of human CHRAC1 (NP_059140.1).
Sequence MADVVVGKDKGGEQRLISLPLSRIRVIMKSSPEVSSINQEALVLTAKATELFVQCLATYSYRHGSGKEKKVLTYSDLANTAQQSETFQFLADILPKKILASKYLKMLKEEKREEDEENDNDNESDHDEADS
Gene ID 54108
Swiss prot Q9NRG0
Synonyms YCL1; CHARC1; CHARC15; CHRAC-1; CHRAC15; CHRAC-15; CHRAC1
Calculated MW 15kDa
Observed MW 15kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples A549
Cellular location Nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

CHRAC1 Rabbit pAb images

ABclonal:Western blot - CHRAC1 Rabbit pAb (A7372)}

Western blot - CHRAC1 Rabbit pAb (A7372)

Western blot analysis of lysates from A549 cells using CHRAC1 Rabbit pAb(A7372) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on CHRAC1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CHRAC1. (Distance between topics and target gene indicate popularity.) CHRAC1

* Data provided by citexs.com, for reference only.

Publishing research using A7372? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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