Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ADAMTS4 Rabbit pAb (A2525)

Publications (9) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - ADAMTS4 Rabbit pAb (A2525)

Western blot analysis of lysates from Mouse heart using ADAMTS4 Rabbit pAb(A2525) at 1:500 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime:3s.

ABclonal:Western blot - ADAMTS4 Rabbit pAb (A2525)

Western blot analysis of lysates from Rat uterus using ADAMTS4 Rabbit pAb(A2525) at 1:500 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Enhanced Kit (RM00021).
Exposuretime:120s.

ABclonal:Immunofluorescence - ADAMTS4 Rabbit pAb (A2525)

Immunofluorescence analysis of SH-SY5Y cells using ADAMTS4 Rabbit pAb (A2525) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name ADAMTS4 Rabbit pAb
Catalog No. A2525
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of this family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The expression of this gene is upregulated in arthritic disease and this may contribute to disease progression through the degradation of aggrecan. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 74-318 of human ADAMTS4 (NP_005090.3).
Sequence GSGAPARLLCRLQAFGETLLLELEQDSGVQVEGLTVQYLGQAPELLGGAEPGTYLTGTINGDPESVASLHWDGGALLGVLQYRGAELHLQPLEGGTPNSAGGPGAHILRRKSPASGQGPMCNVKAPLGSPSPRPRRAKRFASLSRFVETLVVADDKMAAFHGAGLKRYLLTVMAAAAKAFKHPSIRNPVSLVVTRLVILGSGEEGPQVGPSAAQTLRSFCAWQRGLNTPEDSDPDHFDTAILFTR
Gene ID 9507
Swiss prot O75173
Synonyms ADMP-1; ADAMTS-2; ADAMTS-4; ADAMTS4
Calculated MW 90kDa
Observed MW 90kDa/

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples C6, Mouse liver, Mouse testis, Mouse brain, Rat brain, Rat uterus, Mouse heart
Cellular location Secreted, extracellular matrix, extracellular space
Customer validation

WB (Mus musculus, Rattus norvegicus, Homo sapiens)

IF (Sus scrofa)

IHC (Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

ADAMTS4 Rabbit pAb images

ABclonal:Western blot - ADAMTS4 Rabbit pAb (A2525)}

Western blot - ADAMTS4 Rabbit pAb (A2525)

Western blot analysis of lysates from Mouse heart using ADAMTS4 Rabbit pAb(A2525) at 1:500 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime:3s.
ABclonal:Western blot - ADAMTS4 Rabbit pAb (A2525)}

Western blot - ADAMTS4 Rabbit pAb (A2525)

Western blot analysis of lysates from Rat uterus using ADAMTS4 Rabbit pAb(A2525) at 1:500 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Enhanced Kit (RM00021).
Exposuretime:120s.
ABclonal:Immunofluorescence - ADAMTS4 Rabbit pAb (A2525)}

Immunofluorescence - ADAMTS4 Rabbit pAb (A2525)

Immunofluorescence analysis of SH-SY5Y cells using ADAMTS4 Rabbit pAb (A2525) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on ADAMTS4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ADAMTS4. (Distance between topics and target gene indicate popularity.) ADAMTS4

* Data provided by citexs.com, for reference only.

Publishing research using A2525? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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