Product Type > Antibodies > Tag and Loading Control Antibodies

β-Tubulin Mouse mAb (AC021)

Publications (65) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Hamster, Monkey, Goat, Chlamydomonas Reinhardtii

ABclonal:Western blot - β-Tubulin Mouse mAb (AC021)

Western blot analysis of various lysates using β-Tubulin Mouse mAb (AC021) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - β-Tubulin Mouse mAb (AC021)

Immunohistochemistry analysis of β-Tubulin in paraffin-embedded rat ovary using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - β-Tubulin Mouse mAb (AC021)

Immunohistochemistry analysis of β-Tubulin in paraffin-embedded rat stomach using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of C6 cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of NIH-3T3 cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of U-2 OS cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name β-Tubulin Mouse mAb
Catalog No. AC021
Host species Mouse
Purification method Affinity purification
Isotype IgG1
CloneNo. AMC0498

Background

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Immunogen information

Immunogen Recombinant protein of human β-Tubulin
Sequence Email for sequence
Gene ID 203068
Swiss prot P07437
Synonyms M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56; β-Tubulin
Calculated MW 50kDa
Observed MW 50kDa

Applications

Reactivity Human, Mouse, Rat, Hamster, Monkey, Goat, Chlamydomonas Reinhardtii
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:5000 - 1:10000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 1% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples SH-SY5Y, MCF-7, HepG2, HeLa, Mouse brain, Mouse lung, Rat brain
Cellular location Cytoplasm, cytoskeleton
Customer validation

WB (Mus musculus, Homo sapiens, Rattus norvegicus, Arabidopsis thaliana, Crassostrea gigas, Mesocricetus auratus, Danio rerio)

IP (Mus musculus)

IF (Mus musculus, Homo sapiens)

ICH (Mus musculus)

Co-IP (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

β-Tubulin Mouse mAb images

ABclonal:Western blot - β-Tubulin Mouse mAb (AC021)}

Western blot - β-Tubulin Mouse mAb (AC021)

Western blot analysis of various lysates using β-Tubulin Mouse mAb (AC021) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - β-Tubulin Mouse mAb (AC021)}

Immunohistochemistry - β-Tubulin Mouse mAb (AC021)

Immunohistochemistry analysis of β-Tubulin in paraffin-embedded rat ovary using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - β-Tubulin Mouse mAb (AC021)}

Immunohistochemistry - β-Tubulin Mouse mAb (AC021)

Immunohistochemistry analysis of β-Tubulin in paraffin-embedded rat stomach using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021)}

Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of C6 cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021)}

Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of NIH-3T3 cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021)}

Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of U-2 OS cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on TUBB. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TUBB. (Distance between topics and target gene indicate popularity.) TUBB

* Data provided by citexs.com, for reference only.

Publishing research using AC021? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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