Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)
Western blot analysis of lysates from HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)
Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AS014) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Western blot - RRM1 Rabbit pAb (A1152)
Western blot analysis of various lysates using RRM1 Rabbit pAb (A1152) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot - RRM1 Rabbit pAb (A1152)
Western blot analysis of lysates from Rat lung, using RRM1 Rabbit pAb (A1152) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Immunohistochemistry - RRM1 Rabbit pAb (A1152)
Immunohistochemistry analysis of RRM1 in paraffin-embedded human colon using RRM1 Rabbit pAb (A1152) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - RRM1 Rabbit pAb (A1152)
Immunofluorescence analysis of HeLa cells using RRM1 Rabbit pAb (A1152) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation - RRM1 Rabbit pAb (A1152)
Immunoprecipitation analysis of 200 μg extracts of HT-29 cells using 3 μg RRM1 antibody (A1152). Western blot was performed from the immunoprecipitate using RRM1 antibody (A1152) at a dilution of 1:1000.