Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)
Western blot analysis of lysates from HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)
Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AS014) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Western blot - PIDD Rabbit pAb (A4831)
Western blot analysis of extracts of various cell lines, using PIDD antibody (A4831) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry - PIDD Rabbit pAb (A4831)
Immunohistochemistry analysis of paraffin-embedded rat brain using PIDD antibody (A4831) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - PIDD Rabbit pAb (A4831)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using PIDD antibody (A4831) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - PIDD Rabbit pAb (A4831)
Immunohistochemistry analysis of paraffin-embedded mouse brain using PIDD antibody (A4831) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - PIDD Rabbit pAb (A4831)
Immunofluorescence analysis of HeLa cells using PIDD antibody (A4831) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.