Products > Catalog Antibodies > Antibody Duos > Phosphorylated Antibody Duos

Phospho-Stat3-Y705 Antibody Kit (RK04099)

Review (1)Publications (0) Datasheet

ABclonal:Western blot - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunofluorescence - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunofluorescence - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192)
ABclonal:Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192)
ABclonal:Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192)
ABclonal:Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192)

Overview

Product namePhospho-Stat3-Y705 Antibody Kit
Catalog No.RK04099

Product Component

Product nameCatalog No.Positive ApplicationsSpecies Reactivity
Phospho-Stat3-Y705 pAbAP0070WBIHCICCIFIPChIPChIPseqRIPFCHuman, Mouse, Rat
[KO Validated] STAT3 Polyclonal AntibodyA1192WBIHCICCIFIPChIPChIPseqRIPFCHuman, Mouse, Rat, Monkey
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
ImmunogenA synthetic phosphorylated peptide around Y705 of human Stat3 (NP_644805.1).
Recombinant fusion protein containing a sequence corresponding to amino acids 640-770 of human STAT3 (NP_644805.1).
SequenceAPYLK
Gene ID6774
Swiss protP40763
SynonymsADMIO; ADMIO1; APRF; HIES; ADMIO; APRF; HIES
Calculated MW83kDa/87kDa/88kDa
Observed MW105kDa
Storage bufferStore at -20℃. Avoid freeze/ thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular locationCytoplasm, Nucleus
Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Western blot - Phospho-Stat3-Y705 pAb (AP0070) }
Western blot - Phospho-Stat3-Y705 pAb (AP0070)

Western blot analysis of extracts of HeLa cells, using Phospho-Stat3-Y705 antibody (AP0070) at 1:2000 dilution or STAT3 antibody (A1192). HeLa cells were treated by IFN-α (100ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded rat lung using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded rat liver using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded rat testis using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded rat brain using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded rat spleen using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded rat heart using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded human tonsil using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded human lung using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded human liver using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded human appendix using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded human placenta using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded mouse liver using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded mouse brain using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }
Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded mouse pancreas using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - Phospho-Stat3-Y705 pAb (AP0070) }
Immunofluorescence - Phospho-Stat3-Y705 pAb (AP0070)

Immunofluorescence analysis of rat brain using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-Stat3-Y705 pAb (AP0070) }
Immunofluorescence - Phospho-Stat3-Y705 pAb (AP0070)

Immunofluorescence analysis of mouse brain using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.


[KO Validated] STAT3 Polyclonal Antibody (A1192)
ABclonal:Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192) }
Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192)

Western blot analysis of extracts of various cell lines, using STAT3 antibody (A1192) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192) }
Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192)

Western blot analysis of extracts from normal (control) and STAT3 knockout (KO) HeLa cells, using STAT3 antibody (A1192) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192) }
Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192)

Immunofluorescence analysis of rat brain using STAT3 antibody (A1192) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192) }
Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192)

Immunofluorescence analysis of mouse brain using STAT3 antibody (A1192) at dilution of 1:100. Blue: DAPI for nuclear staining.

Submit an review for RK04099

  1. Please choose your application, enter the information requested below. All fields are mandatory, except the ones marked as optional.
  2. When you're happy with the review, submit it by clicking on the Submit button.
1:  
H(s)     M(s)
H(s)      M(s)     Second(s)
(allowed file types: .jpg, .png, .tif, .bmp, .gif)
kDa

Reviews List

Inquire About This Product

Submit your question about RK04099 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
Add to Cart

or Request quote for bulk purchase

Available for next day delivery (US only)

Contact us to order