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Phospho-STAT1-Y701 Antibody Kit (RK04100)

Publication (0) Datasheet

ABclonal:Western blot - Phospho-STAT1-Y701 pAb (AP0135)
ABclonal:Western blot - STAT1 Polyclonal Antibody (A12075)
ABclonal:Immunohistochemistry - STAT1 Polyclonal Antibody (A12075)
ABclonal:Immunohistochemistry - STAT1 Polyclonal Antibody (A12075)
ABclonal:Immunofluorescence - STAT1 Polyclonal Antibody (A12075)
ABclonal:Immunoprecipitation - STAT1 Polyclonal Antibody (A12075)

Overview

Product namePhospho-STAT1-Y701 Antibody Kit
Catalog No.RK04100

Product Component

Product nameCatalog No.Positive ApplicationsSpecies Reactivity
Phospho-STAT1-Y701 pAbAP0135WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman
STAT1 Polyclonal AntibodyA12075WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman, Mouse
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
ImmunogenA phospho specific peptide corresponding to residues surrounding Y701 of human STAT1
A synthetic peptide corresponding to a sequence within amino acids 650-750 of human STAT1 (NP_009330.1).
SequenceEmail for sequence
Gene ID6772
Swiss protP42224
SynonymsCANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
Calculated MW83kDa/87kDa
Observed MW85kDa
Storage bufferStore at -20℃. Avoid freeze/ thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular locationCytoplasm, Nucleus
Phospho-STAT1-Y701 pAb (AP0135)
ABclonal:Western blot - Phospho-STAT1-Y701 pAb (AP0135) }
Western blot - Phospho-STAT1-Y701 pAb (AP0135)

Western blot analysis of extracts of HeLa cells, using Phospho-STAT1-Y701 antibody (AP0135) at 1:1000 dilution or STAT1 antibody (A12075). HeLa cells were treated by IFN-α (100ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.


STAT1 Polyclonal Antibody (A12075)
ABclonal:Western blot - STAT1 Polyclonal Antibody (A12075) }
Western blot - STAT1 Polyclonal Antibody (A12075)

Western blot analysis of extracts of HeLa cells, using STAT1 antibody (A12075) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - STAT1 Polyclonal Antibody (A12075) }
Immunohistochemistry - STAT1 Polyclonal Antibody (A12075)

Immunohistochemistry of paraffin-embedded human mammary cancer using STAT1 antibody (A12075) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - STAT1 Polyclonal Antibody (A12075) }
Immunohistochemistry - STAT1 Polyclonal Antibody (A12075)

Immunohistochemistry of paraffin-embedded human gastric cancer using STAT1 antibody (A12075) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - STAT1 Polyclonal Antibody (A12075) }
Immunofluorescence - STAT1 Polyclonal Antibody (A12075)

Immunofluorescence analysis of U2OS cells using STAT1 antibody (A12075) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - STAT1 Polyclonal Antibody (A12075) }
Immunoprecipitation - STAT1 Polyclonal Antibody (A12075)

Immunoprecipitation analysis of 100ug extracts of HeLa cells using 3ug STAT1 antibody (A12075). Western blot was performed from the immunoprecipitate using STAT1 antibody (A12075) at a dilition of 1:1000.

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