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Phospho-RPS6-S240/244 Antibody Kit (RK04081)

Publication (0) Datasheet

ABclonal:Western blot - Phospho-RPS6-S240/244 pAb (AP0537)
ABclonal:Immunoprecipitation - Phospho-RPS6-S240/244 pAb (AP0537)
ABclonal:Western blot - RPS6 Polyclonal Antibody (A6058)

Overview

Product namePhospho-RPS6-S240/244 Antibody Kit
Catalog No.RK04081

Product Component

Product nameCatalog No.Positive ApplicationsSpecies Reactivity
Phospho-RPS6-S240/244 pAbAP0537WBIHCICCIFIPChIPChIPseqRIPFCHuman, Mouse
RPS6 Polyclonal AntibodyA6058WBIHCICCIFIPChIPChIPseqRIPFCHuman, Mouse, Rat
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
ImmunogenA phospho specific peptide corresponding to residues surrounding S240/244 of human RPS6KB1
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPS6 (NP_001001.2).
SequenceEmail for sequence
Gene ID6194
Swiss protP62753
SynonymsS6
Calculated MW28kDa
Observed MW36kDa
Storage bufferStore at -20℃. Avoid freeze/ thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular location
Phospho-RPS6-S240/244 pAb (AP0537)
ABclonal:Western blot - Phospho-RPS6-S240/244 pAb (AP0537) }
Western blot - Phospho-RPS6-S240/244 pAb (AP0537)

Western blot analysis of extracts of various cell lines, using Phospho-RPS6KB1-S240/244 antibody (AP0537) at 1:1000 dilution or RPS6 antibody (A6058). HeLa cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunoprecipitation - Phospho-RPS6-S240/244 pAb (AP0537) }
Immunoprecipitation - Phospho-RPS6-S240/244 pAb (AP0537)

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-RPS6-S240/244 pAb (AP0537). Western blot was performed from the immunoprecipitate using Phospho-RPS6-S240/244 pAb (AP0537) at a dilition of 1:1000. 293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.


RPS6 Polyclonal Antibody (A6058)
ABclonal:Western blot - RPS6 Polyclonal Antibody (A6058) }
Western blot - RPS6 Polyclonal Antibody (A6058)

Western blot analysis of extracts of various cell lines, using RPS6 antibody (A6058) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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