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Phospho-RELA-S311 Antibody Kit (RK04086)

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ABclonal:Western blot - Phospho-NF-kB p65-S311 pAb (AP0445)
ABclonal:Immunohistochemistry - Phospho-NF-kB p65-S311 pAb (AP0445)
ABclonal:Western blot - [KO Validated] NF-kB p65 Polyclonal Antibody (A11201)
ABclonal:Western blot - [KO Validated] NF-kB p65 Polyclonal Antibody (A11201)
ABclonal:Immunohistochemistry - [KO Validated] NF-kB p65 Polyclonal Antibody (A11201)
ABclonal:Immunofluorescence - [KO Validated] NF-kB p65 Polyclonal Antibody (A11201)

Overview

Product namePhospho-RELA-S311 Antibody Kit
Catalog No.RK04086

Product Component

Product nameCatalog No.Positive ApplicationsSpecies Reactivity
Phospho-NF-kB p65-S311 pAbAP0445WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman, Mouse
[KO Validated] NF-kB p65 Polyclonal AntibodyA11201WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman, Mouse
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around S311 of human RELA (NP_068810.3).
A synthetic peptide corresponding to a sequence within amino acids 450-550 of human RELA (NP_068810.3).
SequenceFKSIM
Gene ID5970
Swiss protQ04206
SynonymsNFKB3; p65
Calculated MW58kDa/59kDa/60kDa
Observed MW60kDa
Storage bufferStore at -20℃. Avoid freeze/ thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular locationCytoplasm, Nucleus
Phospho-NF-kB p65-S311 pAb (AP0445)
ABclonal:Western blot - Phospho-NF-kB p65-S311 pAb (AP0445) }
Western blot - Phospho-NF-kB p65-S311 pAb (AP0445)

Western blot analysis of extracts of HeLa cells, using Phospho-NF-kB p65-S311 antibody (AP0445) at 1:1000 dilution or RELA antibody (A11201). HeLa cells were treated by IFN-α (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Phospho-NF-kB p65-S311 pAb (AP0445) }
Immunohistochemistry - Phospho-NF-kB p65-S311 pAb (AP0445)

Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-NF-kB p65-S311 antibody (AP0445) at dilution of 1:100 (40x lens).


[KO Validated] NF-kB p65 Polyclonal Antibody (A11201)
ABclonal:Western blot - [KO Validated] NF-kB p65 Polyclonal Antibody (A11201) }
Western blot - [KO Validated] NF-kB p65 Polyclonal Antibody (A11201)

Western blot analysis of extracts of HeLa cells, using NF-kB p65 antibody (A11201) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5s.

ABclonal:Western blot - [KO Validated] NF-kB p65 Polyclonal Antibody (A11201) }
Western blot - [KO Validated] NF-kB p65 Polyclonal Antibody (A11201)

Western blot analysis of extracts from normal (control) and NF-kB p65 knockout (KO) HeLa cells, using NF-kB p65 antibody (A11201) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KO Validated] NF-kB p65 Polyclonal Antibody (A11201) }
Immunohistochemistry - [KO Validated] NF-kB p65 Polyclonal Antibody (A11201)

Immunohistochemistry of paraffin-embedded human breast cancer using NF-kB p65 Antibody (A11201) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - [KO Validated] NF-kB p65 Polyclonal Antibody (A11201) }
Immunofluorescence - [KO Validated] NF-kB p65 Polyclonal Antibody (A11201)

Immunofluorescence analysis of A549 cells using NF-kB p65 antibody (A11201) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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