Products > Catalog Antibodies > Antibody Duos > Phosphorylated Antibody Duos

Phospho-PLCG2-Y759 Antibody Kit (RK04095)

Datasheet

ABclonal:Western blot - Phospho-PLCG2-Y759 pAb (AP0785)
ABclonal:Western blot - PLCG2 Polyclonal Antibody (A2182)
ABclonal:Immunohistochemistry - PLCG2 Polyclonal Antibody (A2182)
ABclonal:Immunofluorescence - PLCG2 Polyclonal Antibody (A2182)

Overview

Product namePhospho-PLCG2-Y759 Antibody Kit
Catalog No.RK04095

Product Component

Product nameCatalog No.Positive ApplicationsSpecies Reactivity
Phospho-PLCG2-Y759 pAbAP0785WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman
PLCG2 Polyclonal AntibodyA2182WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman, Mouse
The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4, 5-bisphosphate to 1D-myo-inositol 1, 4, 5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.
ImmunogenA synthetic phosphorylated peptide around Y759 of human PLCG2 (NP_002652.2).
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PLCG2 (NP_002652.2).
SequenceRMYVD
Gene ID5336
Swiss protP16885
SynonymsAPLAID; FCAS3; PLC-gamma-2; PLC-IV
Calculated MW147kDa
Observed MW150kDa
Storage bufferStore at -20℃. Avoid freeze/ thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular location
Phospho-PLCG2-Y759 pAb (AP0785)
ABclonal:Western blot - Phospho-PLCG2-Y759 pAb (AP0785) }
Western blot - Phospho-PLCG2-Y759 pAb (AP0785)

Western blot analysis of extracts of Raji cells, using Phospho-PLCG2-Y759 antibody (AP0785) at 1:2000 dilution or PLCG2 antibody (A2182). Raji cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.


PLCG2 Polyclonal Antibody (A2182)
ABclonal:Western blot - PLCG2 Polyclonal Antibody (A2182) }
Western blot - PLCG2 Polyclonal Antibody (A2182)

Western blot analysis of extracts of various cell lines, using PLCG2 antibody (A2182) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - PLCG2 Polyclonal Antibody (A2182) }
Immunohistochemistry - PLCG2 Polyclonal Antibody (A2182)

Immunohistochemistry of paraffin-embedded mouse heart using PLCG2 antibody (A2182) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - PLCG2 Polyclonal Antibody (A2182) }
Immunofluorescence - PLCG2 Polyclonal Antibody (A2182)

Immunofluorescence analysis of U2OS cells using PLCG2 antibody (A2182).

Inquire About This Product

Submit your question about RK04095 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
Contact us to order