Products > Catalog Antibodies > Antibody Duos > Phosphorylated Antibody Duos

Phospho-PLCG1-S1248 Antibody Kit (RK04096)

Datasheet

ABclonal:Western blot - Phospho-PLCG1-S1248 pAb (AP0827)
ABclonal:Immunohistochemistry - Phospho-PLCG1-S1248 pAb (AP0827)
ABclonal:Western blot - PLCG1 Polyclonal Antibody (A15704)
ABclonal:Immunofluorescence - PLCG1 Polyclonal Antibody (A15704)

Overview

Product namePhospho-PLCG1-S1248 Antibody Kit
Catalog No.RK04096

Product Component

Product nameCatalog No.Positive ApplicationsSpecies Reactivity
Phospho-PLCG1-S1248 pAbAP0827WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman, Mouse, Rat
PLCG1 Polyclonal AntibodyA15704WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman, Mouse, Rat
The protein encoded by this gene catalyzes the formation of inositol 1, 4, 5-trisphosphate and diacylglycerol from phosphatidylinositol 4, 5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around S1248 of human PLCG1 (NP_002651.2).
Recombinant fusion protein containing a sequence corresponding to amino acids 1202-1291 of human PLCG1 (NP_002651.2).
SequenceREGSF
Gene ID5335
Swiss protP19174
SynonymsNCKAP3; PLC-II; PLC1; PLC148; PLCgamma1
Calculated MW148kDa
Observed MW149kDa
Storage bufferStore at -20℃. Avoid freeze/ thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular locationCell projection, lamellipodium, ruffle
Phospho-PLCG1-S1248 pAb (AP0827)
ABclonal:Western blot - Phospho-PLCG1-S1248 pAb (AP0827) }
Western blot - Phospho-PLCG1-S1248 pAb (AP0827)

Western blot analysis of extracts of Jurkat cells, using Phospho-PLCG1-S1248 antibody (AP0827) at dilution or PLCG1 antibody (A15704). Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Phospho-PLCG1-S1248 pAb (AP0827) }
Immunohistochemistry - Phospho-PLCG1-S1248 pAb (AP0827)

Immunohistochemistry of paraffin-embedded human placenta using Phospho-PLCG1-S1248 antibody (AP0827) at dilution of 1:100 (40x lens).


PLCG1 Polyclonal Antibody (A15704)
ABclonal:Western blot - PLCG1 Polyclonal Antibody (A15704) }
Western blot - PLCG1 Polyclonal Antibody (A15704)

Western blot analysis of extracts of various cell lines, using PLCG1 antibody (A15704) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - PLCG1 Polyclonal Antibody (A15704) }
Immunofluorescence - PLCG1 Polyclonal Antibody (A15704)

Immunofluorescence analysis of U2OS cells using PLCG1 antibody (A15704) at dilution of 1:100. Blue: DAPI for nuclear staining.

Submit an review for RK04096

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about RK04096 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
Add to Cart

or Request quote for bulk purchase

Available for next day delivery (US only)

Contact us to order