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Phospho-MAP2K1-S298 Antibody Kit (RK04078)

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ABclonal:Western blot - Phospho-MAP2K1-S298 pAb (AP0063)
ABclonal:Western blot - Phospho-MAP2K1-S298 pAb (AP0063)
ABclonal:Immunoprecipitation - Phospho-MAP2K1-S298 pAb (AP0063)
ABclonal:Western blot - [KO Validated] MAP2K1 Polyclonal Antibody (A12687)
ABclonal:Western blot - [KO Validated] MAP2K1 Polyclonal Antibody (A12687)

Overview

Product namePhospho-MAP2K1-S298 Antibody Kit
Catalog No.RK04078

Product Component

Product nameCatalog No.Positive ApplicationsSpecies Reactivity
Phospho-MAP2K1-S298 pAbAP0063WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman, Mouse, Rat
[KO Validated] MAP2K1 Polyclonal AntibodyA12687WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman, Mouse, Rat
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
ImmunogenA phospho specific peptide corresponding to residues surrounding S298 of human MAP2K1
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP2K1 (NP_002746.1).
SequenceEmail for sequence
Gene ID5604
Swiss protQ02750
SynonymsCFC3; MAPKK1; MEK1; MKK1; PRKMK1
Calculated MW40kDa/43kDa
Observed MW43kDa
Storage bufferStore at -20℃. Avoid freeze/ thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular locationCytoplasm, Membrane, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center, spindle pole body
Phospho-MAP2K1-S298 pAb (AP0063)
ABclonal:Western blot - Phospho-MAP2K1-S298 pAb (AP0063) }
Western blot - Phospho-MAP2K1-S298 pAb (AP0063)

Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-S298 antibody (AP0063) at 1:1000 dilution or MAP2K1 antibody (A12687). HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight or treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Phospho-MAP2K1-S298 pAb (AP0063) }
Western blot - Phospho-MAP2K1-S298 pAb (AP0063)

Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-S298 antibody (AP0063) at 1:1000 dilution. C2C12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or treated by ATP(5 mM) at 30℃ for 1 hour. C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunoprecipitation - Phospho-MAP2K1-S298 pAb (AP0063) }
Immunoprecipitation - Phospho-MAP2K1-S298 pAb (AP0063)

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-MAP2K1-S298 pAb (AP0063). Western blot was performed from the immunoprecipitate using Phospho-MAP2K1-S298 pAb (AP0063) at a dilition of 1:1000. 293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.


[KO Validated] MAP2K1 Polyclonal Antibody (A12687)
ABclonal:Western blot - [KO Validated] MAP2K1 Polyclonal Antibody (A12687) }
Western blot - [KO Validated] MAP2K1 Polyclonal Antibody (A12687)

Western blot analysis of extracts of various cell lines, using MAP2K1 antibody (A12687) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] MAP2K1 Polyclonal Antibody (A12687) }
Western blot - [KO Validated] MAP2K1 Polyclonal Antibody (A12687)

Western blot analysis of extracts from normal (control) and MAP2K1 knockout (KO) HeLa cells, using MAP2K1 antibody (A12687) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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