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Phospho-GRIA1-S845 Antibody Kit (RK04093)

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ABclonal:Western blot - Phospho-GRIA1-S845 pAb (AP0825)
ABclonal:Western blot - GRIA1 Polyclonal Antibody (A1826)
ABclonal:Immunofluorescence - GRIA1 Polyclonal Antibody (A1826)
ABclonal:Immunofluorescence - GRIA1 Polyclonal Antibody (A1826)

Overview

Product namePhospho-GRIA1-S845 Antibody Kit
Catalog No.RK04093

Product Component

Product nameCatalog No.Positive ApplicationsSpecies Reactivity
Phospho-GRIA1-S845 pAbAP0825WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman, Mouse, Rat
GRIA1 Polyclonal AntibodyA1826WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman, Mouse, Rat
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenA phospho specific peptide corresponding to residues surrounding S845 of human GRIA1
Recombinant fusion protein containing a sequence corresponding to amino acids 19-300 of human GRIA1 (NP_000818.2).
SequenceEmail for sequence
Gene ID2890
Swiss protP42261
SynonymsGluA1; GLUH1; GLUR1; GLURA; HBGR1
Calculated MW92kDa/93kDa/101kDa/102kDa
Observed MW90kDa
Storage bufferStore at -20℃. Avoid freeze/ thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular locationCell junction, Cell membrane, Cell projection, Endoplasmic reticulum membrane, Multi-pass membrane protein, dendrite, dendritic spine, postsynaptic cell membrane, postsynaptic density, synapse
Phospho-GRIA1-S845 pAb (AP0825)
ABclonal:Western blot - Phospho-GRIA1-S845 pAb (AP0825) }
Western blot - Phospho-GRIA1-S845 pAb (AP0825)

Western blot analysis of extracts of SH-SY5Y cells, using Phospho-GRIA1-S845 antibody (AP0825) at 1:1000 dilution or GRIA1 antibody (A1826). SH-SY5Y cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.


GRIA1 Polyclonal Antibody (A1826)
ABclonal:Western blot - GRIA1 Polyclonal Antibody (A1826) }
Western blot - GRIA1 Polyclonal Antibody (A1826)

Western blot analysis of extracts of BT-474 cells, using GRIA1 antibody (A1826) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - GRIA1 Polyclonal Antibody (A1826) }
Immunofluorescence - GRIA1 Polyclonal Antibody (A1826)

Immunofluorescence analysis of rat brain using GRIA1 antibody (A1826) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GRIA1 Polyclonal Antibody (A1826) }
Immunofluorescence - GRIA1 Polyclonal Antibody (A1826)

Immunofluorescence analysis of mouse brain using GRIA1 antibody (A1826) at dilution of 1:100. Blue: DAPI for nuclear staining.

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