Products > Catalog Antibodies > Antibody Duos > Phosphorylated Antibody Duos

Phospho-EGFR-Y1045 Antibody Kit (RK04080)

Reviews (2) Datasheet

ABclonal:Western blot - Phospho-EGFR-Y1045 pAb (AP0819)
ABclonal:Western blot - [KO Validated] EGFR Polyclonal Antibody (A11351)
ABclonal:Western blot - [KO Validated] EGFR Polyclonal Antibody (A11351)
ABclonal:Immunohistochemistry - [KO Validated] EGFR Polyclonal Antibody (A11351)
ABclonal:Immunohistochemistry - [KO Validated] EGFR Polyclonal Antibody (A11351)
ABclonal:Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351)
ABclonal:Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351)
ABclonal:Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351)
ABclonal:Immunoprecipitation - [KO Validated] EGFR Polyclonal Antibody (A11351)

Overview

Product namePhospho-EGFR-Y1045 Antibody Kit
Catalog No.RK04080

Product Component

Product nameCatalog No.Positive ApplicationsSpecies Reactivity
Phospho-EGFR-Y1045 pAbAP0819WBIHCICCIFIPChIPChIPseqRIPFCHuman
[KO Validated] EGFR Polyclonal AntibodyA11351WBIHCICCIFIPChIPChIPseqRIPFCHuman, Mouse, Rat
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
ImmunogenA phospho specific peptide corresponding to residues surrounding Y1045 of human EGFR
Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
SequenceEmail for sequence
Gene ID1956
Swiss protP00533
SynonymsERBB; ERBB1; HER1; mENA; NISBD2; PIG61
Calculated MW44kDa/69kDa/77kDa/134kDa
Observed MW180kDa
Storage bufferStore at -20℃. Avoid freeze/ thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular locationCell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein
Phospho-EGFR-Y1045 pAb (AP0819)
ABclonal:Western blot - Phospho-EGFR-Y1045 pAb (AP0819) }
Western blot - Phospho-EGFR-Y1045 pAb (AP0819)

Western blot analysis of extracts of A-431 cells, using Phospho-EGFR-Y1045 antibody (AP0819) at 1:1000 dilution or EGFR antibody (A11351). A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.


[KO Validated] EGFR Polyclonal Antibody (A11351)
ABclonal:Western blot - [KO Validated] EGFR Polyclonal Antibody (A11351) }
Western blot - [KO Validated] EGFR Polyclonal Antibody (A11351)

Western blot analysis of extracts of various cell lines, using EGFR antibody (A11351) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - [KO Validated] EGFR Polyclonal Antibody (A11351) }
Western blot - [KO Validated] EGFR Polyclonal Antibody (A11351)

Western blot analysis of extracts from normal (control) and EGFR knockout (KO) HeLa cells, using EGFR antibody (A11351) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KO Validated] EGFR Polyclonal Antibody (A11351) }
Immunohistochemistry - [KO Validated] EGFR Polyclonal Antibody (A11351)

Immunohistochemistry of paraffin-embedded human prostate using EGFR antibody (A11351) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] EGFR Polyclonal Antibody (A11351) }
Immunohistochemistry - [KO Validated] EGFR Polyclonal Antibody (A11351)

Immunohistochemistry of paraffin-embedded mouse brain using EGFR antibody (A11351) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351) }
Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351)

Immunofluorescence analysis of HeLa cells using EGFR antibody (A11351) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351) }
Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351)

Immunofluorescence analysis of NIH/3T3 cells using EGFR antibody (A11351) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351) }
Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351)

Immunofluorescence analysis of PC12 cells using EGFR antibody (A11351) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] EGFR Polyclonal Antibody (A11351) }
Immunoprecipitation - [KO Validated] EGFR Polyclonal Antibody (A11351)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug EGFR antibody (A11351). Western blot was performed from the immunoprecipitate using EGFR antibody (A11351) at a dilition of 1:1000.

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