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p53 Polyclonal Antibody (A3185)

Reviews (2)Publication (1) Datasheet

ABclonal:Western blot - p53 Polyclonal Antibody (A3185)
ABclonal:Western blot - p53 Polyclonal Antibody (A3185)
ABclonal:Immunohistochemistry - p53 Polyclonal Antibody (A3185)
ABclonal:Immunohistochemistry - p53 Polyclonal Antibody (A3185)
ABclonal:Immunohistochemistry - p53 Polyclonal Antibody (A3185)
ABclonal:Immunofluorescence - p53 Polyclonal Antibody (A3185)
ABclonal:Immunoprecipitation - p53 Polyclonal Antibody (A3185)
Review
ABclonal: review for p53 Polyclonal Antibody(A3185)
Review
ABclonal: review for p53 Polyclonal Antibody(A3185)

Overview

Product namep53 Polyclonal Antibody
Catalog No.A3185
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
ImmunogenRecombinant protein of human p53
SequenceEmail for sequence
Gene ID7157
Swiss protP04637
SynonymsBCC7; LFS1; P53; TRP53; p53; TP53; BMFS5
Calculated MW23-43kDa
Observed MW53kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesSW480, MCF-7, U-251MG, Mouse thymus
Cellular locationCytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Customer validation

(, )

WB(Human)

ABclonal:Western blot - p53 Polyclonal Antibody (A3185) }

Western blot - p53 Polyclonal Antibody (A3185)

Western blot analysis of extracts of various cell lines, using p53 antibody (A3185) at 1:300 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - p53 Polyclonal Antibody (A3185) }

Western blot - p53 Polyclonal Antibody (A3185)

Western blot analysis of extracts of Mouse thymus, using p53 antibody (A3185) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - p53 Polyclonal Antibody (A3185) }

Immunohistochemistry - p53 Polyclonal Antibody (A3185)

Immunohistochemistry of paraffin-embedded human gastric cancer using p53 Antibody (A3185) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - p53 Polyclonal Antibody (A3185) }

Immunohistochemistry - p53 Polyclonal Antibody (A3185)

Immunohistochemistry of paraffin-embedded human B cell lymphoma using p53 Antibody (A3185) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - p53 Polyclonal Antibody (A3185) }

Immunohistochemistry - p53 Polyclonal Antibody (A3185)

Immunohistochemistry of paraffin-embedded human gastric cancer using p53 Antibody (A3185) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - p53 Polyclonal Antibody (A3185) }

Immunofluorescence - p53 Polyclonal Antibody (A3185)

Immunofluorescence analysis of MCF-7 cells using p53 antibody (A3185).
ABclonal:Immunoprecipitation - p53 Polyclonal Antibody (A3185) }

Immunoprecipitation - p53 Polyclonal Antibody (A3185)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug p53 antibody (A3185). Western blot was performed from the immunoprecipitate using p53 antibody (A3185) at a dilition of 1:1000.

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