Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

[One Step] Hsp60 Antibody Kit (RK05661)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:

ABclonal:Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Western blot analysis of lysates from HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AS014) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Western blot - HSP60/HSPD1 Rabbit pAb (A0969)

Western blot analysis of various lysates using HSP60/HSPD1 Rabbit pAb (A0969) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - HSP60/HSPD1 Rabbit pAb (A0969)

Immunohistochemistry analysis of HSP60/HSPD1 in paraffin-embedded rat kidney using HSP60/HSPD1 Rabbit pAb (A0969) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - HSP60/HSPD1 Rabbit pAb (A0969)

Immunohistochemistry analysis of HSP60/HSPD1 in paraffin-embedded human colon using HSP60/HSPD1 Rabbit pAb (A0969) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - HSP60/HSPD1 Rabbit pAb (A0969)

Immunohistochemistry analysis of HSP60/HSPD1 in paraffin-embedded mouse testis using HSP60/HSPD1 Rabbit pAb (A0969) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - HSP60/HSPD1 Rabbit pAb (A0969)

Confocal immunofluorescence analysis of HeLa cells using HSP60/HSPD1 Rabbit pAb (A0969) at dilution of 1:400. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HSP60/HSPD1 Rabbit pAb (A0969)

Confocal immunofluorescence analysis of U2OS cells using HSP60/HSPD1 Rabbit pAb (A0969) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - HSP60/HSPD1 Rabbit pAb (A0969)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 1 μg HSP60/HSPD1 antibody (A0969). Western blot was performed from the immunoprecipitate using HSP60/HSPD1 antibody (A0969) at a dilution of 1:1000.

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Overview

Product name [One Step] Hsp60 Antibody Kit
Catalog No. RK05661
Host species
Purification method
Isotype

Background

Immunogen information

Immunogen
Sequence Email for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW

Applications

Reactivity
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
Storage buffer
Application key
Positive samples
Cellular location

[One Step] Hsp60 Antibody Kit images

ABclonal:Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)}

Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Western blot analysis of lysates from HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)}

Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AS014) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Western blot - HSP60/HSPD1 Rabbit pAb (A0969)}

Western blot - HSP60/HSPD1 Rabbit pAb (A0969)

Western blot analysis of various lysates using HSP60/HSPD1 Rabbit pAb (A0969) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - HSP60/HSPD1 Rabbit pAb (A0969)}

Immunohistochemistry - HSP60/HSPD1 Rabbit pAb (A0969)

Immunohistochemistry analysis of HSP60/HSPD1 in paraffin-embedded rat kidney using HSP60/HSPD1 Rabbit pAb (A0969) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - HSP60/HSPD1 Rabbit pAb (A0969)}

Immunohistochemistry - HSP60/HSPD1 Rabbit pAb (A0969)

Immunohistochemistry analysis of HSP60/HSPD1 in paraffin-embedded human colon using HSP60/HSPD1 Rabbit pAb (A0969) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - HSP60/HSPD1 Rabbit pAb (A0969)}

Immunohistochemistry - HSP60/HSPD1 Rabbit pAb (A0969)

Immunohistochemistry analysis of HSP60/HSPD1 in paraffin-embedded mouse testis using HSP60/HSPD1 Rabbit pAb (A0969) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - HSP60/HSPD1 Rabbit pAb (A0969)}

Immunofluorescence - HSP60/HSPD1 Rabbit pAb (A0969)

Confocal immunofluorescence analysis of HeLa cells using HSP60/HSPD1 Rabbit pAb (A0969) at dilution of 1:400. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HSP60/HSPD1 Rabbit pAb (A0969)}

Immunofluorescence - HSP60/HSPD1 Rabbit pAb (A0969)

Confocal immunofluorescence analysis of U2OS cells using HSP60/HSPD1 Rabbit pAb (A0969) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - HSP60/HSPD1 Rabbit pAb (A0969)}

Immunoprecipitation - HSP60/HSPD1 Rabbit pAb (A0969)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 1 μg HSP60/HSPD1 antibody (A0969). Western blot was performed from the immunoprecipitate using HSP60/HSPD1 antibody (A0969) at a dilution of 1:1000.

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Related Targets

Based on existing publications, the following genes are closely related to research on HSPD1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HSPD1. (Distance between topics and target gene indicate popularity.) HSPD1

* Data provided by citexs.com, for reference only.

Publishing research using RK05661? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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