Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)
Western blot analysis of lysates from HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)
Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AS014) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Western blot - HSP60/HSPD1 Rabbit pAb (A0969)
Western blot analysis of various lysates using HSP60/HSPD1 Rabbit pAb (A0969) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry - HSP60/HSPD1 Rabbit pAb (A0969)
Immunohistochemistry analysis of HSP60/HSPD1 in paraffin-embedded rat kidney using HSP60/HSPD1 Rabbit pAb (A0969) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry - HSP60/HSPD1 Rabbit pAb (A0969)
Immunohistochemistry analysis of HSP60/HSPD1 in paraffin-embedded human colon using HSP60/HSPD1 Rabbit pAb (A0969) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry - HSP60/HSPD1 Rabbit pAb (A0969)
Immunohistochemistry analysis of HSP60/HSPD1 in paraffin-embedded mouse testis using HSP60/HSPD1 Rabbit pAb (A0969) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence - HSP60/HSPD1 Rabbit pAb (A0969)
Confocal immunofluorescence analysis of HeLa cells using HSP60/HSPD1 Rabbit pAb (A0969) at dilution of 1:400. Blue: DAPI for nuclear staining.
Immunofluorescence - HSP60/HSPD1 Rabbit pAb (A0969)
Confocal immunofluorescence analysis of U2OS cells using HSP60/HSPD1 Rabbit pAb (A0969) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation - HSP60/HSPD1 Rabbit pAb (A0969)
Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 1 μg HSP60/HSPD1 antibody (A0969). Western blot was performed from the immunoprecipitate using HSP60/HSPD1 antibody (A0969) at a dilution of 1:1000.