Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ZC3H12A Rabbit pAb (A12667)

Publications (4) Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - ZC3H12A Rabbit pAb (A12667)

Western blot analysis of various lysates using ZC3H12A Rabbit pAb (A12667) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name ZC3H12A Rabbit pAb
Catalog No. A12667
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

ZC3H12A is an MCP1 (CCL2; MIM 158105)-induced protein that acts as a transcriptional activator and causes cell death of cardiomyocytes, possibly via induction of genes associated with apoptosis.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human ZC3H12A (NP_079355.2).
Sequence MSGPCGEKPVLEASPTMSLWEFEDSHSRQGTPRPGQELAAEEASALELQMKVDFFRKLGYSSTEIHSVLQKLGVQADTNTVLGELVKHGTATERERQTSPDPCPQLPLVPRGGGTPKAPNLEPPLPEEEKEGSDLRPVVI
Gene ID 80149
Swiss prot Q5D1E8
Synonyms Reg1; MCPIP; MCPIP1; MCPIP-1; dJ423B22.1; ZC3H12A
Calculated MW 66kDa
Observed MW 66kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:3000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples A-549, Raji, Jurkat
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Sus scrofa, Homo sapiens, Mus musculus)

Research Area

ZC3H12A Rabbit pAb images

ABclonal:Western blot - ZC3H12A Rabbit pAb (A12667)}

Western blot - ZC3H12A Rabbit pAb (A12667)

Western blot analysis of various lysates using ZC3H12A Rabbit pAb (A12667) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ZC3H12A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ZC3H12A. (Distance between topics and target gene indicate popularity.) ZC3H12A

* Data provided by citexs.com, for reference only.

Publishing research using A12667? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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