Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

p53 Rabbit pAb (A3185)

Reviews (2) Publications (16) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - p53 Rabbit pAb (A3185)

Western blot analysis of various lysates using p53 Rabbit pAb (A3185) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - p53 Rabbit pAb (A3185)

Western blot analysis of lysates from NIH/3T3 cells, using p53 Rabbit pAb (A3185) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - p53 Rabbit pAb (A3185)

Immunohistochemistry analysis of p53 in paraffin-embedded human colon carcinoma using p53 Rabbit pAb (A3185) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - p53 Rabbit pAb (A3185)

Immunofluorescence analysis of MCF-7 cells using p53 Rabbit pAb (A3185).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

ABclonal:Immunofluorescence - p53 Rabbit pAb (A3185)

Immunofluorescence analysis of HeLa cells using p53 Rabbit pAb (A3185) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - p53 Rabbit pAb (A3185)

Immunofluorescence analysis of NIH/3T3 cells using p53 Rabbit pAb (A3185) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - p53 Rabbit pAb (A3185)

Immunofluorescence analysis of C6 cells using p53 Rabbit pAb (A3185) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - p53 Rabbit pAb (A3185)

Immunoprecipitation analysis of 200 μg extracts of U-251MG cells using 3 μg p53 Rabbit pAb (A3185). Western blot was performed from the immunoprecipitate using p53 Rabbit pAb (A3185) at a dilition of 1:1000.

Review
ABclonal: review for p53 Rabbit pAb(A3185)
Review
ABclonal: review for p53 Rabbit pAb(A3185)

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Overview

Product name p53 Rabbit pAb
Catalog No. A3185
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-393 of human p53 (NP_000537.3).
Sequence MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD
Gene ID 7157
Swiss prot P04637
Synonyms P53; BCC7; LFS1; BMFS5; TRP53; p53
Calculated MW 44kDa
Observed MW 53kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples U-251MG, MCF7, 293T, NIH/3T3
Cellular location Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Customer validation

WB (U2OS, Non-small-cell carcinomaHomo sapiens, Sanghuangporus vaninii, Rattus norvegicus, Homo sapiens, Mus musculus, Aedes aegypti)

IHC (Mus musculus, Rattus norvegicus)

qRT-PCR (Mus musculus)

IF (Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

p53 Rabbit pAb images

ABclonal:Western blot - p53 Rabbit pAb (A3185)}

Western blot - p53 Rabbit pAb (A3185)

Western blot analysis of various lysates using p53 Rabbit pAb (A3185) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - p53 Rabbit pAb (A3185)}

Western blot - p53 Rabbit pAb (A3185)

Western blot analysis of lysates from NIH/3T3 cells, using p53 Rabbit pAb (A3185) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - p53 Rabbit pAb (A3185)}

Immunohistochemistry - p53 Rabbit pAb (A3185)

Immunohistochemistry analysis of p53 in paraffin-embedded human colon carcinoma using p53 Rabbit pAb (A3185) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - p53 Rabbit pAb (A3185)}

Immunofluorescence - p53 Rabbit pAb (A3185)

Immunofluorescence analysis of MCF-7 cells using p53 Rabbit pAb (A3185).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.
ABclonal:Immunofluorescence - p53 Rabbit pAb (A3185)}

Immunofluorescence - p53 Rabbit pAb (A3185)

Immunofluorescence analysis of HeLa cells using p53 Rabbit pAb (A3185) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - p53 Rabbit pAb (A3185)}

Immunofluorescence - p53 Rabbit pAb (A3185)

Immunofluorescence analysis of NIH/3T3 cells using p53 Rabbit pAb (A3185) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - p53 Rabbit pAb (A3185)}

Immunofluorescence - p53 Rabbit pAb (A3185)

Immunofluorescence analysis of C6 cells using p53 Rabbit pAb (A3185) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - p53 Rabbit pAb (A3185)}

Immunoprecipitation - p53 Rabbit pAb (A3185)

Immunoprecipitation analysis of 200 μg extracts of U-251MG cells using 3 μg p53 Rabbit pAb (A3185). Western blot was performed from the immunoprecipitate using p53 Rabbit pAb (A3185) at a dilition of 1:1000.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on TP53. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TP53. (Distance between topics and target gene indicate popularity.) TP53

* Data provided by citexs.com, for reference only.

Publishing research using A3185? Please let us know so that we can cite the reference in this datasheet.

Antibodies (31)

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Secondary Antibodies (23)

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